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Western Blot (WB) (Western blot analysis of PAX1 expression in Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, The lane on the left is treated with the antigen-specific peptide.)

Rabbit anti-Human, Mouse PAX1 Polyclonal Antibody | anti-PAX1 antibody

PAX1 Antibody

Gene Names
PAX1; OFC2; HUP48
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PAX1; Polyclonal Antibody; PAX1 Antibody; HUP48; OTTHUMP00000030407; Paired box 1; Paired box gene 1; Paired box protein Pax 1; Paired box protein Pax-1; Paired domain gene HuP48; PAX 1; Pax1; PAX1_HUMAN; anti-PAX1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
PAX1 antibody detects endogenous levels of total PAX1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
534
Applicable Applications for anti-PAX1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (89%), Xenopus (89%)
Conjugation
Unconjugated

Western Blot (WB)

(Western blot analysis of PAX1 expression in Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, The lane on the left is treated with the antigen-specific peptide.)

Western Blot (WB) (Western blot analysis of PAX1 expression in Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, The lane on the left is treated with the antigen-specific peptide.)

Western Blot (WB)

(Western blot analysis of extracts from Hela, using PAX1 Antibody. Lane 1 was treated with the blocking peptide.)

Western Blot (WB) (Western blot analysis of extracts from Hela, using PAX1 Antibody. Lane 1 was treated with the blocking peptide.)

Immunohistochemistry (IHC)

(MBS9604118 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9604118 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-PAX1 antibody
Function: This protein is a transcriptional activator. It may play a role in the formation of segmented structures of the embryo. May play an important role in the normal development of the vertebral column (By similarity).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 55 kDa
Predicted: 56 kDa
NCBI Official Full Name
paired box protein Pax-1 isoform 1
NCBI Official Synonym Full Names
paired box 1
NCBI Official Symbol
PAX1
NCBI Official Synonym Symbols
OFC2; HUP48
NCBI Protein Information
paired box protein Pax-1
UniProt Protein Name
Paired box protein Pax-1
Protein Family
UniProt Gene Name
PAX1
UniProt Synonym Gene Names
HUP48

NCBI Description

This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. This gene plays a role in pattern formation during embryogenesis and may be essential for development of the vertebral column. This gene is silenced by methylation in ovarian and cervical cancers and may be a tumor suppressor gene. Mutations in this gene are also associated with vertebral malformations. [provided by RefSeq, Mar 2012]

Uniprot Description

This protein is a transcriptional activator. It may play a role in the formation of segmented structures of the embryo. May play an important role in the normal development of the vertebral column ().

Research Articles on PAX1

Similar Products

Product Notes

The PAX1 pax1 (Catalog #AAA9604118) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAX1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PAX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the PAX1 pax1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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