Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Formin-binding protein 1 (FNBP1) Standard Curve.)
Rat Formin-Binding Protein 1 (FNBP1) ELISA Kit | FNBP1 elisa kit
Rat Formin-Binding Protein 1 (FNBP1) ELISA Kit
Reactivity
Rat
Synonyms
Formin-Binding Protein 1 (FNBP1); Rat Formin-Binding Protein 1 (FNBP1) ELISA Kit; FNBP1; FBP17; KIAA0554; MGC126804; formin-binding protein 17; FNBP1 elisa kit
Reactivity
Rat
Specificity
Rat Formin-binding protein 1 (FNBP1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat FNBP1. No significant cross-reactivity or interference between Rat FNBP1 and analogues was observed.
Sequence Length
620
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Formin-binding protein 1 (FNBP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Formin-binding protein 1 (FNBP1) Standard Curve.)
Related Product Information for FNBP1 elisa kit
Description: This Rat Formin-binding protein 1 (FNBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate FNBP1 in samples. An antibody specific for FNBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FNBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FNBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FNBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Formin-binding protein 1 is a member of the formin-binding-protein family. The protein contains an N-terminal Fer/Cdc42-interacting protein 4 (CIP4) homology (FCH) domain followed by a coiled-coil domain, a proline-rich motif, a second coiled-coil domain, a Rho family protein-binding domain (RBD), and a C-terminal SH3 domain. This protein binds sorting nexin 2 (SNX2), tankyrase (TNKS), and dynamin; an interaction between this protein and formin has not been demonstrated yet in human. Very highly expressed in the epithelial cells of the gastrointestinal tract, respiratory, reproductive and urinary systems. Also highly expressed in brown adipose tissue, cardiomyocytes, enteric ganglia and glucagon producing cells of the pancreas. Expressed in germ cells of the testis and all regions of the brain.
Background: Formin-binding protein 1 is a member of the formin-binding-protein family. The protein contains an N-terminal Fer/Cdc42-interacting protein 4 (CIP4) homology (FCH) domain followed by a coiled-coil domain, a proline-rich motif, a second coiled-coil domain, a Rho family protein-binding domain (RBD), and a C-terminal SH3 domain. This protein binds sorting nexin 2 (SNX2), tankyrase (TNKS), and dynamin; an interaction between this protein and formin has not been demonstrated yet in human. Very highly expressed in the epithelial cells of the gastrointestinal tract, respiratory, reproductive and urinary systems. Also highly expressed in brown adipose tissue, cardiomyocytes, enteric ganglia and glucagon producing cells of the pancreas. Expressed in germ cells of the testis and all regions of the brain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
formin-binding protein 1
NCBI Official Synonym Full Names
formin binding protein 1
NCBI Official Symbol
Fnbp1
NCBI Protein Information
formin-binding protein 1
UniProt Protein Name
Formin-binding protein 1
Protein Family
UniProt Gene Name
Fnbp1
UniProt Synonym Gene Names
Fbp17
UniProt Entry Name
FNBP1_RAT
NCBI Description
member of the Rho family of GTPases, binds effector protein rapostlin and regulates neurite branch formation [RGD, Feb 2006]
Research Articles on FNBP1
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Product Notes
The Rat FNBP1 fnbp1 (Catalog #AAA9712580) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712580 ELISA Kit recognizes Rat FNBP1. It is sometimes possible for the material contained within the vial of "Formin-Binding Protein 1 (FNBP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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