Rabbit TRAF4 Polyclonal Antibody | anti-TRAF4 antibody

Anti-TRAF4 antibody

Cat. Num. AAA176312

• Gene Names: TRAF4; CART1; MLN62; RNF83
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: TRAF4; Polyclonal Antibody; Anti-TRAF4 antibody; TNF receptor-associated factor 4; CART 1 antibody; CART1 antibody; cysteine rich domain associated with ring and TRAF domain antibody; Cysteine rich domain associated with RING and Traf domains protein 1 antibody; Cysteine-rich domain associated with RING and Traf domains protein 1 antibody; Malignant 62 antibody; metastatic lymph node gene 62 protein antibody; MLN 62 antibody; MLN62 antibody; RING finger protein 83 antibody; RNF 83 antibody; RNF83 antibody; TNF receptor associated factor 4 antibody; TNF receptor-associated factor 4 antibody; TRAF 4 antibody; Traf4 antibody; TRAF4_HUMAN antibody; tumor necrosis receptor-associated factor 4A antibody; anti-TRAF4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 470

• Applicable Applications for anti-TRAF4 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TRAF4 (437-454aa PKFISHQDIRKRNYVRDD), identical to the related rat and mouse sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-TRAF4 antibody, MBS176312, Western blottingLane 1: Rat Thymus Tissue LysateLane 2: HELA Cell LysateLane 3: JURKAT Cell LysateLane 4: HEPA Cell Lysate )

Immunohistochemistry (IHC)

(Anti-TRAF4 antibody, MBS176312, IHC(P)IHC(P): Rat Lymphonodus Tissue )

Immunohistochemistry (IHC)

(Anti-TRAF4 antibody, MBS176312, IHC(P)IHC(P): Human Lung Cancer Tissue )

Related Product Information for anti-TRAF4 antibody

Description: Rabbit IgG polyclonal antibody for TNF receptor-associated factor 4(TRAF4) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: TRAF4(TNF Receptor-Associated Factor 4), also called CART1 or MLN62, is a protein that in humans is encoded by the TRAF3 gene. TRAF4 is a member of the TNF receptor associated factor(TRAF) family, a family of scaffold proteins. Tomasetto et al.(1995) identified genes that are overexpressed in breast cancer by differential screening of cDNAs from breast cancer-derived metastatic axillary lymph nodes. The TRAF4 cDNA, which they designated MLN62, was mapped to 17q11-q12 by radioisotopic in situ hybridization. To investigate the biologic function of TRAF4, Regnier et al.(2002) generated Traf4-deficient mice by gene disruption.

NCBI and Uniprot Product Information

• NCBI GI #: 30580636
• NCBI GeneID: 9618
• UniProt Accession #: Q9BUZ4; O75615; Q14848; Q2KJU4; Q2PJN8
• Molecular Weight: 22,841 Da
• NCBI Official Full Name: TNF receptor-associated factor 4
• NCBI Official Synonym Full Names: TNF receptor-associated factor 4
• NCBI Official Symbol: TRAF4
• NCBI Official Synonym Symbols: CART1; MLN62; RNF83
• NCBI Protein Information: TNF receptor-associated factor 4; MLN 62; malignant 62; RING finger protein 83; metastatic lymph node gene 62 protein; tumor necrosis receptor-associated factor 4A; cysteine-rich domain associated with ring and TRAF domain; cysteine-rich domain associated with RING and Traf domains protein 1
• UniProt Protein Name: TNF receptor-associated factor 4
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: TRAF4
• UniProt Synonym Gene Names: CART1; MLN62; RNF83; MLN 62
• UniProt Entry Name: TRAF4_HUMAN

NCBI Description

This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TRAF4: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract. Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. Belongs to the TNF receptor-associated factor family. B subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q11-q12

Cellular Component: cytoskeleton; tight junction; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; thioesterase binding; ubiquitin protein ligase binding; WW domain binding; ubiquitin-protein ligase activity; protein kinase binding; tumor necrosis factor receptor binding

Biological Process: regulation of apoptosis; apoptosis; respiratory tube development; positive regulation of protein kinase activity; activation of NF-kappaB-inducing kinase; positive regulation of JNK cascade; protein ubiquitination; respiratory gaseous exchange; signal transduction