Immunohistochemistry (IHC)
(MBS9603988 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse ZNF71 Polyclonal Antibody | anti-ZNF71 antibody
ZNF71 Antibody
Gene Names
ZNF71; EZFIT
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ZNF71; Polyclonal Antibody; ZNF71 Antibody; Cos26; Endothelial zinc finger protein; Endothelial zinc finger protein induced by tumor necrosis factor alpha; EZFIT; Kruppel related zinc finger protein; Zinc finger protein 71 (Cos26); Zinc finger protein 71; ZNF47; ZNF71_HUMAN; anti-ZNF71 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ZNF71 antibody detects endogenous levels of total ZNF71
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
489
Applicable Applications for anti-ZNF71 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Subcellular Location
Nucleus
Tissue Specificity
Highly expressed in placenta, followed by brain, testis, pancreas, heart, small intestine, muscle, uterus, prostate and peripheral blood leukocytes. Not detected in liver, lung, colon, stomach, salivary and thyroid gland.
Conjugation
Unconjugated
Immunohistochemistry (IHC)
(MBS9603988 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603988 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of ZNF71 expression in A431 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of ZNF71 expression in A431 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-ZNF71 antibody
Description: May be involved in transcriptional regulation.
Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family.
Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 54 kDa
Predicted: 55 kDa
Predicted: 55 kDa
NCBI Official Full Name
endothelial zinc finger protein induced by tumor necrosis factor alpha
NCBI Official Synonym Full Names
zinc finger protein 71
NCBI Official Symbol
ZNF71
NCBI Official Synonym Symbols
EZFIT
NCBI Protein Information
endothelial zinc finger protein induced by tumor necrosis factor alpha
UniProt Protein Name
Endothelial zinc finger protein induced by tumor necrosis factor alpha
UniProt Gene Name
ZNF71
UniProt Synonym Gene Names
EZFIT
Uniprot Description
May be involved in transcriptional regulation.
Research Articles on ZNF71
Similar Products
Product Notes
The ZNF71 znf71 (Catalog #AAA9603988) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZNF71 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF71 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the ZNF71 znf71 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZNF71, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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