Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Testing Data (P-peptide-+)

anti-Human, Mouse 14-3-3 theta/tau Antibody | anti-14-3-3 theta/tau antibody

14-3-3 theta/tau (Phospho-Ser232) Antibody

Gene Names
YWHAQ; 1C5; HS1; 14-3-3
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
14-3-3 theta/tau; 14-3-3 theta/tau (Phospho-Ser232) Antibody; 14-3-3 protein T-cell; 14-3-3 protein tau; 1433T; 143T; HS1 protein; YWHAQ; anti-14-3-3 theta/tau antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Specificity
14-3-3 theta/tau (Phospho-Ser232) antibody detects endogenous levels of 14-3-3 theta/tau only when phosphorylated at serine 232.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
245
Applicable Applications for anti-14-3-3 theta/tau antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 theta/tau around the phosphorylation site of serine 232 (S-D-SP-A-G).
All Sites
Human: Ser232
Mouse: Ser232
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(P-peptide-+)

Testing Data (P-peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using 14-3-3 theta/tau (Phospho-Ser232) antibody. Western blot analysis of extracts from HeLa cells, using 14-3-3 theta/tau (Phospho-Ser232) antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using 14-3-3 theta/tau (Phospho-Ser232) antibody. Western blot analysis of extracts from HeLa cells, using 14-3-3 theta/tau (Phospho-Ser232) antibody.)

Immunofluorescence (IF)

(P-peptide-+ Immunofluorescence analysis of HeLa cells, using 14-3-3 theta/tau (Phospho-Ser232) antibody.)

Immunofluorescence (IF) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using 14-3-3 theta/tau (Phospho-Ser232) antibody.)
References
Nielsen P.J., im. Biophys. Acta 1088:425-428(1991). Leffers H., J. Mol. Biol. 231:982-998(1993). Gevaert K., Nat. Biotechnol. 21:566-569(2003).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,764 Da
NCBI Official Full Name
14-3-3 protein theta
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
NCBI Official Symbol
YWHAQ
NCBI Official Synonym Symbols
1C5; HS1; 14-3-3
NCBI Protein Information
14-3-3 protein theta
UniProt Protein Name
14-3-3 protein theta
UniProt Gene Name
YWHAQ

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]

Uniprot Description

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

Research Articles on 14-3-3 theta/tau

Similar Products

Product Notes

The 14-3-3 theta/tau ywhaq (Catalog #AAA859994) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The 14-3-3 theta/tau (Phospho-Ser232) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3 theta/tau can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the 14-3-3 theta/tau ywhaq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 theta/tau, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.