Rabbit OTUD7B Polyclonal Antibody | anti-OTUD7B antibody

OTUD7B Antibody

Cat. Num. AAA9603446

• Gene Names: OTUD7B; ZA20D1; CEZANNE
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: OTUD7B; Polyclonal Antibody; OTUD7B Antibody; Cellular zinc finger anti NF kappa B protein; Cellular zinc finger anti-NF-kappa-B protein; CEZANNE; HGNC:16683; OTU domain containing protein 7B; OTU domain-containing protein 7B; OTU7B_HUMAN; ZA20D1; Zinc finger A20 domain containing protein 1; Zinc finger A20 domain-containing protein 1; Zinc finger protein Cezanne; anti-OTUD7B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: OTUD7B antibody detects endogenous levels of OTUD7B.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 843

• Applicable Applications for anti-OTUD7B antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human OTUD7B.
• Subcellular Location: Cytoplasm. Nucleus.
• Tissue Specificity: Widely expressed. Abundant in kidney, heart and fetal liver. Expressed differentially among B-cells at distinct developmental stages. Higher expression seen in primary immature B-cells as compared to the mature cells.
• Predicted Cross Reactivity: Pig, Bovine, Sheep, Dog
• Similarity: Pig (100%), Bovine (100%), Sheep (100%), Dog (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts of Jurkat cells, using OTUD7B antibody.)

Immunohistochemistry (IHC)

(MBS9603446 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-OTUD7B antibody

Function: Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections (By similarity). Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241). Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2 (By similarity). Mediates deubiquitination of EGFR (PubMed:22179831). Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains (PubMed:27732584). Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro); however the physiological significance of these data are unsure (PubMed:27732584). Hydrolyzes both linear and branched forms of polyubiquitin.
Subunit Structure: Interacts with ZAP70 in activated T cells, but not in resting T cells (PubMed:26903241). Interacts with TRAF3 (By similarity). Interacts with TRAF6 (PubMed:11463333). Interacts with PARK7, leading to inhibit deubiquitinase activity (PubMed:21097510). Interacts with EGFR, ITCH and NEDD4 (PubMed:22179831).
Post-translational Modifications: Phosphorylated by EGFR.
Similarity: The protein undergoes a significant conformation change upon binding to ubiquitinated substrates. The loop that precedes the active site is in an autoinhibitory conformation in the apoprotein. Ubiquitin binding leads to a conformation change; the loop is stabilized in a catalytically competent conformation with the result that the active site Cys can form the reaction state intermediate. Belongs to the peptidase C64 family.

NCBI and Uniprot Product Information

• NCBI GI #: 118026942
• NCBI GeneID: 56957
• NCBI Accession #: NP_064590.2
• NCBI GenBank Nucleotide #: NM_020205.3
• UniProt Accession #: Q6GQQ9; Q5SZ60; Q8WWA7; Q9NQ53; Q9UFF4; B7Z643; D3DUZ8
• Molecular Weight: Observed: 100 kDa; Predicted: 93 kDa
• NCBI Official Full Name: OTU domain-containing protein 7B
• NCBI Official Synonym Full Names: OTU deubiquitinase 7B
• NCBI Official Symbol: OTUD7B
• NCBI Official Synonym Symbols: ZA20D1; CEZANNE
• NCBI Protein Information: OTU domain-containing protein 7B
• UniProt Protein Name: OTU domain-containing protein 7B
• Protein Family: OTU domain-containing protein
• UniProt Gene Name: OTUD7B
• UniProt Synonym Gene Names: ZA20D1; Cezanne3 PublicationsManual assertion based on opinion iniRef.1

Uniprot Description

Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections (). Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241). Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2 (). Mediates deubiquitination of EGFR (PubMed:22179831). Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains (PubMed:27732584). Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro); however the physiological significance of these data are unsure (PubMed:27732584). Hydrolyzes both linear and branched forms of polyubiquitin.