Western Blot (WB)
(OTU7B Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the OTU7B antibody detected the OTU7B protein (arrow).)
Rabbit OTU7B Polyclonal Antibody | anti-OTUD7B antibody
OTU7B Antibody (C-term)
Gene Names
OTUD7B; ZA20D1; CEZANNE
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
OTU7B; Polyclonal Antibody; OTU7B Antibody (C-term); OTU domain-containing protein 7B; Cellular zinc finger anti-NF-kappa-B protein; Zinc finger A20 domain-containing protein 1; Zinc finger protein Cezanne; OTUD7B; ZA20D1; anti-OTUD7B antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This OTU7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 757-784 amino acids from the C-terminal region of human OTU7B.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
757-784
Sequence Length
814
Applicable Applications for anti-OTUD7B antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(OTU7B Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the OTU7B antibody detected the OTU7B protein (arrow).)
Western Blot (WB)
(OTU7B Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the OTU7B antibody detected the OTU7B protein (arrow).)
Immunohistochemistry (IHC)
(OTU7B Antibody (C-term) (MBS9201330)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OTU7B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(OTU7B Antibody (C-term) (MBS9201330)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OTU7B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-OTUD7B antibody
OTU7B has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. Hydrolyzes both linear and branched forms of polyubiquitin. Negative regulator of nuclear factor NF-kappa-B.
Product Categories/Family for anti-OTUD7B antibody
References
Bremm, A., et al. Nat. Struct. Mol. Biol. 17(8):939-947(2010)
Jin, Z., et al. Cell 137(4):721-735(2009)
Enesa, K., et al. J. Biol. Chem. 283(27):18582-18590(2008)
Bohgaki, M., et al. Biochim. Biophys. Acta 1783(5):826-837(2008)
Enesa, K., et al. J. Biol. Chem. 283(11):7036-7045(2008)
Jin, Z., et al. Cell 137(4):721-735(2009)
Enesa, K., et al. J. Biol. Chem. 283(27):18582-18590(2008)
Bohgaki, M., et al. Biochim. Biophys. Acta 1783(5):826-837(2008)
Enesa, K., et al. J. Biol. Chem. 283(11):7036-7045(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92526
NCBI Official Full Name
OTU domain-containing protein 7B
NCBI Official Synonym Full Names
OTU deubiquitinase 7B
NCBI Official Symbol
OTUD7B
NCBI Official Synonym Symbols
ZA20D1; CEZANNE
NCBI Protein Information
OTU domain-containing protein 7B
UniProt Protein Name
OTU domain-containing protein 7B
Protein Family
UniProt Gene Name
OTUD7B
UniProt Synonym Gene Names
ZA20D1
UniProt Entry Name
OTU7B_HUMAN
Uniprot Description
Cezanne: cellular zinc finger anti-NF-kappaB, a negative regulator of NF-kappaB. A novel deubiquitinating enzyme that belongs to the ovarian tumor protein (OTU) superfamily.
Protein type: EC 3.4.19.12; Ubiquitin conjugating system; Protease
Chromosomal Location of Human Ortholog: 1q21.2
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin-specific protease activity; cysteine-type peptidase activity
Biological Process: mucosal immune response; negative regulation of interleukin-8 production; negative regulation of transcription from RNA polymerase II promoter; negative regulation of I-kappaB kinase/NF-kappaB cascade; proteolysis
Research Articles on OTUD7B
Similar Products
Product Notes
The OTUD7B otud7b (Catalog #AAA9201330) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 757-784. The OTU7B Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's OTU7B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the OTUD7B otud7b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OTU7B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
