Rabbit MMS19 Polyclonal Antibody | anti-MMS19 antibody

MMS19 Antibody

Cat. Num. AAA9603440

• Gene Names: MMS19; CIAO4; MET18; MMS19L; hMMS19
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: MMS19; Polyclonal Antibody; MMS19 Antibody; hMMS19; homolog of yeast MMS19; MET18; MET18 homolog; MET18MMS19 nucleotide excision repair homolog (S. cerevisiae); mms19; MMS19 nucleotide excision repair protein homolog; MMS19 nucleotide excision repair; S. cerevisiae; homolog of; MMS19-like (MET18 homolog; S. cerevisiae); MMS19-like protein; MMS19_HUMAN; MMS19L; anti-MMS19 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MMS19 antibody detects endogenous levels of MMS19.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 987

• Applicable Applications for anti-MMS19 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human MMS19.
• Subcellular Location: Nucleus. Cytoplasm > Cytoskeleton > Spindle.
• Tissue Specificity: Ubiquitously expressed with higher expression in testis.
• Predicted Cross Reactivity: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
• Similarity: Pig (100%), Zebrafish (91%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts of mouse testis tissue, using MMS19 antibody.)

Immunohistochemistry (IHC)

(MBS9603440 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-MMS19 antibody

Function: Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription (PubMed:22678362, PubMed:22678361, PubMed:29225034). As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD (PubMed:20797633). Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery (PubMed:11279242).
Subunit Structure: Component of the CIA complex. Component of the MMXD complex, composed of CIAO1, ERCC2, FAM96B, MMS19 and SLC25A5. Interacts with FAM96B; the interaction is direct. Interacts with ERCC2/XPD; the interaction is direct. Interacts with ERCC3/XPB and NCOA3/RAC3. Interacts with RTEL1; the interaction mediates the association of RTEL1 with the CIA complex.
Post-translational Modifications: Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination by MAGEF1-NSMCE1 ubiquitin ligase complex leading to proteasomal degradation.
Similarity: Belongs to the MET18/MMS19 family.

NCBI and Uniprot Product Information

• NCBI GI #: 574957085
• NCBI GeneID: 64210
• NCBI Accession #: NP_001276332.1
• NCBI GenBank Nucleotide #: NM_001289403.1
• UniProt Accession #: Q96T76; Q17RZ8; Q5T455; Q66K82; Q7L4W8; Q969Z1; B0QZ75; B3KPE5; B4DQX2; B4E2I3; D3DR55; F8W9Y2
• Molecular Weight: Observed: 103-113 kDa; Predicted: 114 kDa
• NCBI Official Full Name: MMS19 nucleotide excision repair protein homolog isoform 2
• NCBI Official Synonym Full Names: MMS19 homolog, cytosolic iron-sulfur assembly component
• NCBI Official Symbol: MMS19
• NCBI Official Synonym Symbols: CIAO4; MET18; MMS19L; hMMS19
• NCBI Protein Information: MMS19 nucleotide excision repair protein homolog
• UniProt Protein Name: MMS19 nucleotide excision repair protein homolog
• Protein Family: MMS19 nucleotide excision repair protein
• UniProt Gene Name: MMS19
• UniProt Synonym Gene Names: hMMS19

Uniprot Description

Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription (PubMed:22678362, PubMed:22678361, PubMed:29225034, PubMed:23585563). As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD (PubMed:20797633). Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery (PubMed:11279242).