Western Blot (WB)
(Figure 1: Western blot analysis using Myostatin mouse mAb against truncated Myostatin-His recombinant protein (1).)
Mouse anti-Human Myostatin Monoclonal Antibody | anti-MSTN antibody
Myostatin Antibody
Gene Names
MSTN; GDF8; MSLHP
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity-Chromatography
Synonyms
Myostatin; Monoclonal Antibody; Myostatin Antibody; GDF 8; GDF-8; GDF8; GDF8_HUMAN; Growth differentiation factor 8; Growth/differentiation factor 8; MSLHP; MSTN; OTTHUMP00000163498; anti-MSTN antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
Myostatin antibody detects endogenous levels of total Myostatin
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
375
Applicable Applications for anti-MSTN antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
WB: 1:500-1:2000
Immunogen
Purified recombinant fragment of human Myostatin expressed in E. Coli
Subcellular Location
Secreted.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using Myostatin mouse mAb against truncated Myostatin-His recombinant protein (1).)
Western Blot (WB)
(Figure 1: Western blot analysis using Myostatin mouse mAb against truncated Myostatin-His recombinant protein (1).)
Related Product Information for anti-MSTN antibody
Description: The protein is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth.
Function: Acts specifically as a negative regulator of skeletal muscle growth.
Subunit Structure: Homodimer; disulfide-linked (PubMed:27625211). Interacts with WFIKKN2, leading to inhibit its activity (PubMed:12595574). Interacts with FST3 (PubMed:17878677).
Post-translational Modifications: Synthesized as large precursor molecule that undergoes proteolytic cleavage to generate an N-terminal propeptide and a disulfide linked C-terminal dimer, which is the biologically active molecule. The circulating form consists of a latent complex of the C-terminal dimer and other proteins, including its propeptide, which maintain the C-terminal dimer in a latent, inactive state. Ligand activation requires additional cleavage of the prodomain by a tolloid-like metalloproteinase.
Similarity: Belongs to the TGF-beta family.
Function: Acts specifically as a negative regulator of skeletal muscle growth.
Subunit Structure: Homodimer; disulfide-linked (PubMed:27625211). Interacts with WFIKKN2, leading to inhibit its activity (PubMed:12595574). Interacts with FST3 (PubMed:17878677).
Post-translational Modifications: Synthesized as large precursor molecule that undergoes proteolytic cleavage to generate an N-terminal propeptide and a disulfide linked C-terminal dimer, which is the biologically active molecule. The circulating form consists of a latent complex of the C-terminal dimer and other proteins, including its propeptide, which maintain the C-terminal dimer in a latent, inactive state. Ligand activation requires additional cleavage of the prodomain by a tolloid-like metalloproteinase.
Similarity: Belongs to the TGF-beta family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 42.7 kDa
Predicted: 43 kDa
Predicted: 43 kDa
NCBI Official Full Name
growth/differentiation factor 8 preproprotein
NCBI Official Synonym Full Names
myostatin
NCBI Official Symbol
MSTN
NCBI Official Synonym Symbols
GDF8; MSLHP
NCBI Protein Information
growth/differentiation factor 8
UniProt Protein Name
Growth/differentiation factor 8
Protein Family
UniProt Gene Name
MSTN
UniProt Synonym Gene Names
GDF8; GDF-8
NCBI Description
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. [provided by RefSeq, Jul 2016]
Uniprot Description
Acts specifically as a negative regulator of skeletal muscle growth.
Research Articles on MSTN
Similar Products
Product Notes
The MSTN mstn (Catalog #AAA9602508) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Myostatin Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Myostatin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the MSTN mstn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myostatin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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