Immunofluorescence (IF)
(MBS9602160 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human MRGF Polyclonal Antibody | anti-MRGF antibody
MRGF Antibody
Gene Names
MRGPRF; RTA; MRGF; GPR140; GPR168
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MRGF; Polyclonal Antibody; MRGF Antibody; DKFZp586B2122; FLJ16111; FLJ29034; FLJ40998; FLJ53714; G protein coupled receptor 140; G protein coupled receptor 168; G protein coupled receptor MrgF; G-protein coupled receptor 140; G-protein coupled receptor 168; GPR140; GPR168; Mas related G protein coupled MRGF; Mas related G protein coupled receptor member F; Mas related gene F protein; MAS related GPR member F; Mas-related G-protein coupled receptor member F; Mas-related gene F protein; MGC21621; Mrgprf; MRGRF_HUMAN; RTA; Seven transmembrane helix receptor; anti-MRGF antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
MRGF antibody detects endogenous levels of total MRGF
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
343
Applicable Applications for anti-MRGF antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IHC: 1:200
IF/ICC: 1:100-1:500
IHC: 1:200
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human MRGF
Subcellular Location
Plasma Membrane
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9602160 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9602160 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of MRGF using HuvEc whole cell lysates)
Western Blot (WB)
(Western blot analysis of MRGF using HuvEc whole cell lysates)
Immunohistochemistry (IHC)
(MBS9602160 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9602160 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-MRGF antibody
Function: Orphan receptor. May bind to a neuropeptide and may regulate nociceptor function and/or development, including the sensation or modulation of pain (By similarity).
Similarity: Belongs to the G-protein coupled receptor 1 family. Mas subfamily.
Similarity: Belongs to the G-protein coupled receptor 1 family. Mas subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 40 kDa
Predicted: 39 kDa
Predicted: 39 kDa
NCBI Official Full Name
mas-related G-protein coupled receptor member F
NCBI Official Synonym Full Names
MAS related GPR family member F
NCBI Official Symbol
MRGPRF
NCBI Official Synonym Symbols
RTA; MRGF; GPR140; GPR168
NCBI Protein Information
mas-related G-protein coupled receptor member F
UniProt Protein Name
Mas-related G-protein coupled receptor member F
UniProt Gene Name
MRGPRF
UniProt Synonym Gene Names
GPR140; GPR168; MRGF; Mas-related gene F protein
Uniprot Description
Orphan receptor. May bind to a neuropeptide and may regulate nociceptor function and/or development, including the sensation or modulation of pain ().
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Product Notes
The MRGF mrgprf (Catalog #AAA9602160) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MRGF Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MRGF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IHC: 1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the MRGF mrgprf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRGF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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