Western Blot (WB)
(Western blot analysis of HMGA1 expression in mouse brain tissue, The lane on the left is treated with the antigen-specific peptide.)
Rabbit HMGA1 Polyclonal Antibody | anti-HMGA1 antibody
HMGA1 Antibody
Gene Names
HMGA1; HMG-R; HMGIY; HMGA1A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
HMGA1; Polyclonal Antibody; HMGA1 Antibody; High mobility group (nonhistone chromosomal) protein isoforms I and Y; High mobility group AT hook 1; High mobility group AT-hook protein 1; High mobility group protein 1; High mobility group protein A1; High mobility group protein HMG-I/HMG-Y; High mobility group protein Ia; High mobility group protein R; HMG R; HMG-I(Y); HMGA1_HUMAN; HMGA1A; HMGIY; MGC12816; MGC4242; MGC4854; Nonhistone chromosomal high mobility group protein HMG I/HMG Y; anti-HMGA1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
HMGA1 antibody detects endogenous levels of total HMGA1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
96
Applicable Applications for anti-HMGA1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human HMGA1
Subcellular Location
Nucleus. Chromosome.
Predicted Cross Reactivity
Pig, Bovine, Horse, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of HMGA1 expression in mouse brain tissue, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of HMGA1 expression in mouse brain tissue, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-HMGA1 antibody
Description: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
Subunit Structure: Interacts with HIPK2 (By similarity). Interacts with HIV-1 pre-integration complex.
Post-translational Modifications: Constitutively phosphorylated on two or three sites. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoforms HMG-I and HMG-Y can be phosphorylated by HIPK2. Phosphorylation of HMG-I at Ser-36, Thr-53 and Thr-78 and of HMG-Y at Thr-42 and Thr-67 by HIPK2 modulates DNA-binding affinity. HMG-Y is not methylated. Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.
Similarity: Belongs to the HMGA family.
Subunit Structure: Interacts with HIPK2 (By similarity). Interacts with HIV-1 pre-integration complex.
Post-translational Modifications: Constitutively phosphorylated on two or three sites. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoforms HMG-I and HMG-Y can be phosphorylated by HIPK2. Phosphorylation of HMG-I at Ser-36, Thr-53 and Thr-78 and of HMG-Y at Thr-42 and Thr-67 by HIPK2 modulates DNA-binding affinity. HMG-Y is not methylated. Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.
Similarity: Belongs to the HMGA family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 11 kDa
Predicted: 12 kDa
Predicted: 12 kDa
NCBI Official Full Name
high mobility group protein HMG-I/HMG-Y isoform b
NCBI Official Synonym Full Names
high mobility group AT-hook 1
NCBI Official Symbol
HMGA1
NCBI Official Synonym Symbols
HMG-R; HMGIY; HMGA1A
NCBI Protein Information
high mobility group protein HMG-I/HMG-Y
UniProt Protein Name
High mobility group protein HMG-I/HMG-Y
Protein Family
UniProt Gene Name
HMGA1
UniProt Synonym Gene Names
HMGIY; HMG-I(Y); High mobility group protein A1
NCBI Description
This gene encodes a chromatin-associated protein involved in the regulation of gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of AT-rich regions in double-stranded DNA. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been identified on multiple chromosomes. [provided by RefSeq, Jan 2016]
Uniprot Description
HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
Research Articles on HMGA1
Similar Products
Product Notes
The HMGA1 hmga1 (Catalog #AAA9601391) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HMGA1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HMGA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the HMGA1 hmga1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMGA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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