Rabbit EPS15 Polyclonal Antibody | anti-EPS15 antibody

Phospho-EPS15(Tyr849) Antibody

Cat. Num. AAA9600026

• Gene Names: EPS15; AF1P; AF-1P; MLLT5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
• Synonyms: EPS15; Polyclonal Antibody; Phospho-EPS15(Tyr849) Antibody; AF 1P; AF 1p protein; AF1P; ALL1 fused gene from chromosome 1; Epidermal growth factor receptor pathway substrate 15; Epidermal growth factor receptor substrate 15; EPS 15; MLLT 5; MLLT5; Protein Eps 15; Protein Eps15; anti-EPS15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-EPS15(Tyr849) antibody detects endogenous levels of EPS15 only when phosphorylated at Tyrosine 849
• Purity/Purification: From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 582

• Applicable Applications for anti-EPS15 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide derived from human EPS15 around the phosphorylation site of Tyrosine 849
• Subcellular Location: Cytoplasm. Cell Membrane; Peripheral Membrane Protein; Cytoplasmic Side. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits.
• Tissue Specificity: Ubiquitously expressed.
• Conjugation: Unconjugated
• Epitope: Phospho Tyr849
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis on HepG2 cell lysate using Phospho-EPS15(Tyr849) Antibody, The lane on the left is treated with the antigen-specific peptide.)

Immunofluorescene (IF)

(MBS9600026 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Related Product Information for anti-EPS15 antibody

Description: This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Function: Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
Subunit Structure: Interacts with SGIP1 (PubMed:26822536). Interacts with HGS; the interaction bridges the interaction of STAM or STAM2 with EPS15. Isoform 2 interacts with HGS and AP2A2. Part of a complex at least composed of EPS15, HGS, and either STAM or STAM2. Binds AP2A2. Interacts with AP2B1; clathrin competes with EPS15. Binds STON2 and EPN1. Interacts (via its SH3-binding sites) with CRK. Interacts with SH3BP4/TTP. Interacts with ERBB2. Interacts with FCHO1. Interacts with FCHO2. Interacts (via EH domains) with DAB2. Interacts (via UIM repeats) with CORO7 (when ubiquitinated at 'Lys-472'). Interacts (via UIM domains) with UBQLN1 (via ubiquitin-like domain) and can interact with both the ubiquitinated and the non-ubiquitinated forms of UBQLN1. Interacts with UBQLN2 (By similarity) (PubMed:10757979, PubMed:11062555, PubMed:12551915, PubMed:16159959, PubMed:16314522, PubMed:16325581, PubMed:16903783, PubMed:18199683, PubMed:18200045, PubMed:18362181, PubMed:20448150, PubMed:21762413, PubMed:22484487, PubMed:22648170, PubMed:24768539, PubMed:8662907, PubMed:9721102, PubMed:9723620). Interacts with vaccinia virus protein A36 (PubMed:27670116).
Post-translational Modifications: Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis (By similarity). Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Ubiquitinated.
Similarity: The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.The UIM (ubiquitin-interacting motif) repeats specifically bind 'Lys-33'-linked ubiquitin.

NCBI and Uniprot Product Information

• NCBI GI #: 229577185
• NCBI GeneID: 2060
• NCBI Accession #: NP_001153441.1
• NCBI GenBank Nucleotide #: NM_001159969.1
• UniProt Accession #: P42566; Q5SRH4; B2R8J7; D3DPJ2
• Molecular Weight: Observed: 98 kDa; Predicted: 99 kDa
• NCBI Official Full Name: epidermal growth factor receptor substrate 15 isoform B
• NCBI Official Synonym Full Names: epidermal growth factor receptor pathway substrate 15
• NCBI Official Symbol: EPS15
• NCBI Official Synonym Symbols: AF1P; AF-1P; MLLT5
• NCBI Protein Information: epidermal growth factor receptor substrate 15
• UniProt Protein Name: Epidermal growth factor receptor substrate 15
• Protein Family: Epidermal growth factor receptor substrate
• UniProt Gene Name: EPS15
• UniProt Synonym Gene Names: AF1P; Protein Eps15

NCBI Description

This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2009]

Uniprot Description

Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.