Human Carbonic Anhydrase IX CLIA Kit | CA9 clia kit

Carbonic Anhydrase IX (Human) LumiAb ELISA Kit

Cat. Num. AAA9510954

• Gene Names: CA9; MN; CAIX
• Reactivity: Human
• Synonyms: Carbonic Anhydrase IX; Carbonic Anhydrase IX (Human) LumiAb ELISA Kit; Human Carbonic Anhydrase IX; Carbonic anhydrase 9; Carbonate dehydratase IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G4-250; pMW1; G4-250; MN; CA9 clia kit

• Reactivity: Human
• Specificity: The LumiAb Human BMP-2 ELISA is capable of recognizing both recombinant and naturally produced Human BMP-2 proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 10% cross reactivity.
• Sequence Length: 459

• Assay Type: Quantitative Sandwich
• Sensitivity: 8 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CA9 clia kit

Principle of the Assay: The LumiAb Human BMP-2 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human BMP-2 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human BMP-2 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human BMP-2, also known as Bone Morphogenetic Protein 2, is a 396 amino acid cytokine protein implicated in the induction of cartilage and bone formation. The protein expressed from the BMP2 gene at locus 20p12 on chromosome 20 belongs to the Transforming Growth Factor beta (TGFbeta) superfamily. After initial translation of the peptide, it is subject to proteolytic processing and cleavage, which results in the separation of the 23 residue signal sequence from the 259 residue pro-peptide and 114 residue mature BMP-2 cytokine. The encoded protein acts as a disulfide-linked homodimer that interacts with SOSTDC1, GREM2, RGMA, RGMB, RGMC and ASPN. In terms of tissue specificity, the BMP-2 protein is particularly abundant in the lungs, spleen and colon and less abundant but still prevalent in the heart, brain, placenta, liver, skeletal muscle, kidneys, pancreas, prostate, ovaries and small intestine.

Product Categories/Family for CA9 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 119578763
• NCBI GeneID: 768
• UniProt Accession #: Q16790
• NCBI Official Full Name: carbonic anhydrase IX
• NCBI Official Synonym Full Names: carbonic anhydrase 9
• NCBI Official Symbol: CA9
• NCBI Official Synonym Symbols: MN; CAIX
• NCBI Protein Information: carbonic anhydrase 9
• UniProt Protein Name: Carbonic anhydrase 9
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA9
• UniProt Synonym Gene Names: G250; MN; CA-IX; CAIX; RCC-associated antigen G250

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

Uniprot Description

Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.