Rabbit B4GALT3 Polyclonal Antibody | anti-B4GALT3 antibody

B4GALT3 Antibody

Cat. Num. AAA9506097

• Gene Names: B4GALT3; beta4Gal-T3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: B4GALT3; Polyclonal Antibody; B4GALT3 Antibody; Beta-1; 4-galactosyltransferase 3; 4-GalTase 3; Beta4Gal-T3; b4Gal-T3; UDP-galactose:beta-N-acetylglucosamine beta-1; UDP-Gal:beta-GlcNAc beta-1; anti-B4GALT3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: B4GALT3 Antibody detects endogenous levels of total B4GALT3 protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 145

• Applicable Applications for anti-B4GALT3 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:40000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human B4GALT3.
• Immunogen Range: 271-320
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using B4GALT3 Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from Jurkat, HeLa, and HepG2 cells, using B4GALT3 Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 315467841
• NCBI GeneID: 8703
• NCBI Accession #: NP_001186802.1
• NCBI GenBank Nucleotide #: NM_001199873.1
• UniProt Accession #: O60512; O60910; Q9BPZ4; Q9H8T2; D3DVG3
• Molecular Weight: 43 kDa
• NCBI Official Full Name: beta-1,4-galactosyltransferase 3
• NCBI Official Synonym Full Names: beta-1,4-galactosyltransferase 3
• NCBI Official Symbol: B4GALT3
• NCBI Official Synonym Symbols: beta4Gal-T3
• NCBI Protein Information: beta-1,4-galactosyltransferase 3
• UniProt Protein Name: Beta-1,4-galactosyltransferase 3
• Protein Family: Beta-1,4-galactosyltransferase
• UniProt Gene Name: B4GALT3
• UniProt Synonym Gene Names: Beta-1,4-GalTase 3; Beta4Gal-T3; b4Gal-T3

NCBI Description

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene encodes an enzyme that may be mainly involved in the synthesis of the first N-acetyllactosamine unit of poly-N-acetyllactosamine chains. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.