Rabbit anti-Human, Mouse CD5L Polyclonal Antibody | anti-CD5L antibody

CD5L Rabbit pAb

Cat. Num. AAA4754786

• Gene Names: CD5L; AIM; API6; CT-2; hAIM; PRO229; Spalpha; SP-ALPHA
• Reactivity: Human, Mouse
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: CD5L; Polyclonal Antibody; CD5L Rabbit pAb; CD5 molecule-like; AIM; API6; PRO229; Spalpha; SP-ALPHA; anti-CD5L antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Expressed in spleen, lymph node, thymus, bone marrow, and fetal liver, but not in non-lymphoid tissues.
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-CD5L antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:1000-1:5000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide of human CD5L
• Conjugation: Unconjugated
• Molecular Function: Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflamed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis. Able to inhibit lipid droplet size in adipocytes. Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA). CD5L-induced lipolysis occurs with progression of obesity: participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease. Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC). Acts as a key regulator of metabolic switch in T-helper Th17 cells. Regulates the expression of pro-inflammatory genes in Th17 cells by altering the lipid content and limiting synthesis of cholesterol ligand of RORC, the master transcription factor of Th17-cell differentiation. CD5L is mainly present in non-pathogenic Th17 cells, where it decreases the content of polyunsaturated fatty acyls (PUFA), affecting two metabolic proteins MSMO1 and CYP51A1, which synthesize ligands of RORC, limiting RORC activity and expression of pro-inflammatory genes. Participates in obesity-associated autoimmunity via its association with IgM, interfering with the binding of IgM to Fcalpha/mu receptor and enhancing the development of long-lived plasma cells that produce high-affinity IgG autoantibodies. Also acts as an inhibitor of apoptosis in macrophages: promotes macrophage survival from the apoptotic effects of oxidized lipids in case of atherosclerosis (PubMed:24295828). Involved in early response to microbial infection against various pathogens by acting as a pattern recognition receptor and by promoting autophagy (PubMed:16030018, PubMed:24223991, PubMed:24583716, PubMed:25713983).
• Modification: Unmodified
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human Lymphoma tissue using 161540(CD5L Antibody) at dilution 1/40. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 161540(CD5L Antibody) at dilution 1/40.(Original magnification: ×200))

Related Product Information for anti-CD5L antibody

CD5L (CD5 molecule-like), also known as API6, PRO229, Spalpha or SP-ALPHA, is a 347 amino acid secreted protein that belongs to the scavenger receptor cysteine-rich (SRCR) family of leukocyte regulating proteins. Expressed in bone marrow, spleen, thymus, lymph node and fetal liver, CD5L is thought to be involved in regulating the immune system via binding to peripheral monocytes and mediating their activation and overall survival. CD5L has three cysteine-rich domains and, in addition to its role in the immune system, may function to inhibit apoptosis and promote macrophage survival.

Product Categories/Family for anti-CD5L antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 5174411
• NCBI GeneID: 922
• NCBI Accession #: NP_005885.1
• NCBI GenBank Nucleotide #: NM_005894.2
• UniProt Accession #: O43866; Q6UX63; A8K7M5
• Molecular Weight: 38,088 Da
• NCBI Official Full Name: CD5 antigen-like
• NCBI Official Synonym Full Names: CD5 molecule like
• NCBI Official Symbol: CD5L
• NCBI Official Synonym Symbols: AIM; API6; CT-2; hAIM; PRO229; Spalpha; SP-ALPHA
• NCBI Protein Information: CD5 antigen-like
• UniProt Protein Name: CD5 antigen-like
• Protein Family: CD5 antigen
• UniProt Gene Name: CD5L
• UniProt Synonym Gene Names: API6; hAIM
• UniProt Entry Name: CD5L_HUMAN

Uniprot Description

CD5L: May play a role in the regulation of the immune system. Seems to play a role as an inhibitor of apoptosis.

Protein type: Receptor, misc.; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: extracellular space

Biological Process: cellular defense response