Western Blot (WB)
(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane: HEPG2 cell Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1MIN)
Rabbit anti-Human DNASE2 Polyclonal Antibody | anti-DNASE2 antibody
DNASE2 Antibody
Gene Names
DNASE2; DNL; DNL2; DNASE2A
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
DNASE2; Polyclonal Antibody; DNASE2 Antibody; DNL; DNL2; DNASE2A; anti-DNASE2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total DNASE2 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
0.7 mg/ml (varies by lot)
Sequence Length
360
Applicable Applications for anti-DNASE2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:30-1:150
Immunohistochemistry: 1:30-1:150
Immunogen
Synthetic peptide of human DNASE2
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane: HEPG2 cell Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1MIN)
Western Blot (WB)
(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane: HEPG2 cell Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1MIN)
Related Product Information for anti-DNASE2 antibody
This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record.
Product Categories/Family for anti-DNASE2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,615 Da
NCBI Official Full Name
deoxyribonuclease-2-alpha
NCBI Official Synonym Full Names
deoxyribonuclease II, lysosomal
NCBI Official Symbol
DNASE2
NCBI Official Synonym Symbols
DNL; DNL2; DNASE2A
NCBI Protein Information
deoxyribonuclease-2-alpha
UniProt Protein Name
Deoxyribonuclease-2-alpha
Protein Family
UniProt Gene Name
DNASE2
UniProt Synonym Gene Names
DNASE2A; DNL2; DNase II alpha
UniProt Entry Name
DNS2A_HUMAN
Uniprot Description
DNASE2: Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. Belongs to the DNase II family.
Protein type: Deoxyribonuclease; EC 3.1.22.1
Chromosomal Location of Human Ortholog: 19p13.2
Molecular Function: DNA binding
Biological Process: DNA fragmentation during apoptosis; DNA metabolic process
Similar Products
Product Notes
The DNASE2 dnase2 (Catalog #AAA9415490) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNASE2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNASE2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:200-1:1000 Immunohistochemistry: 1:30-1:150. Researchers should empirically determine the suitability of the DNASE2 dnase2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNASE2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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