Rabbit anti-Human DNA Polymerase alpha Polyclonal Antibody | anti-POLA1 antibody

DNA Polymerase alpha Antibody

Cat. Num. AAA9504931

• Gene Names: POLA1; NSX; POLA; p180
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: DNA Polymerase alpha; Polyclonal Antibody; DNA Polymerase alpha Antibody; DNA polymerase alpha catalytic subunit; POLA1; POLA; anti-POLA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: DNA Polymerase alpha Antibody detects endogenous levels of total DNA Polymerase alpha protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1,462

• Applicable Applications for anti-POLA1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:20000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human DNA Polymerase alpha.
• Immunogen Range: 81-130
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Western Blot (WB)

(Western blot analysis of lysates from HepG2, 293, and Jurkat cells, using DNA Polymerase alpha Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 106507301
• NCBI GeneID: 5422
• NCBI Accession #: NP_058633.2
• NCBI GenBank Nucleotide #: NM_016937.3
• UniProt Accession #: P09884; Q86UQ7
• Molecular Weight: 165 kDa
• NCBI Official Full Name: DNA polymerase alpha catalytic subunit isoform 2
• NCBI Official Synonym Full Names: DNA polymerase alpha 1, catalytic subunit
• NCBI Official Symbol: POLA1
• NCBI Official Synonym Symbols: NSX; POLA; p180
• NCBI Protein Information: DNA polymerase alpha catalytic subunit
• UniProt Protein Name: DNA polymerase alpha catalytic subunit
• Protein Family: DNA polymerase
• UniProt Gene Name: POLA1
• UniProt Synonym Gene Names: POLA

NCBI Description

This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010]

Uniprot Description

Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses (PubMed:27019227).