Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NEDD8 Antibody. The picture on the right is blocked with the synthesized peptide.)
Rabbit NEDD8 Polyclonal Antibody | anti-NEDD8 antibody
NEDD8 Antibody
Gene Names
NEDD8; NEDD-8
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
NEDD8; Polyclonal Antibody; NEDD8 Antibody; Ubiquitin-like protein Nedd8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; anti-NEDD8 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
NEDD8 Antibody detects endogenous levels of total NEDD8 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
81
Applicable Applications for anti-NEDD8 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:5000
IHC: 1:50~1:100
ELISA: 1:5000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human NEDD8.
Immunogen Range
Oct-59
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NEDD8 Antibody. The picture on the right is blocked with the synthesized peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NEDD8 Antibody. The picture on the right is blocked with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of lysates from Jurkat cells, using NEDD8 Antibody. The lane on the right is blocked with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of lysates from Jurkat cells, using NEDD8 Antibody. The lane on the right is blocked with the synthesized peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
9 kDa
NCBI Official Full Name
NEDD8
NCBI Official Synonym Full Names
neural precursor cell expressed, developmentally down-regulated 8
NCBI Official Symbol
NEDD8
NCBI Official Synonym Symbols
NEDD-8
NCBI Protein Information
NEDD8
UniProt Protein Name
NEDD8
Protein Family
UniProt Gene Name
NEDD8
UniProt Synonym Gene Names
NEDD-8
Uniprot Description
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Research Articles on NEDD8
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Product Notes
The NEDD8 nedd8 (Catalog #AAA9504754) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEDD8 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NEDD8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000. Researchers should empirically determine the suitability of the NEDD8 nedd8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEDD8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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