Human bromodomain containing 4 ELISA Kit | BRD4 elisa kit

Human Bromodomain-containing protein 4, BRD4 ELISA Kit

Cat. Num. AAA945281

• Gene Names: BRD4; CAP; MCAP; HUNK1; HUNKI
• Reactivity: Human
• Synonyms: bromodomain containing 4; Human Bromodomain-containing protein 4; BRD4 ELISA Kit; Human Bromodomain-containing protein 4 (BRD4) ELISA kit; CAP; HUNK1; HUNKI; MCAP; bromodomain-containing 4; bromodomain-containing protein 4; chromosome-associated protein; BRD4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human BRD4. No significant cross-reactivity or interference between human BRD4 and analogues was observed.
• Sequence Length: 722

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 18.75 pg/ml-1200 pg/ml
• Sensitivity: < 4.68 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BRD4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BRD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BRD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BRD4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BRD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 7657218
• NCBI GeneID: 23476
• NCBI Accession #: NP_055114.1
• NCBI GenBank Nucleotide #: NM_014299.2
• UniProt Accession #: O60885; O60433; Q4G0X8; Q86YS8; Q96PD3
• Molecular Weight: 152,219 Da
• NCBI Official Full Name: bromodomain-containing protein 4 isoform short
• NCBI Official Synonym Full Names: bromodomain containing 4
• NCBI Official Symbol: BRD4
• NCBI Official Synonym Symbols: CAP; MCAP; HUNK1; HUNKI
• NCBI Protein Information: bromodomain-containing protein 4; bromodomain-containing 4; chromosome-associated protein
• UniProt Protein Name: Bromodomain-containing protein 4
• Protein Family: Bromodomain-containing protein
• UniProt Gene Name: BRD4
• UniProt Synonym Gene Names: HUNK1
• UniProt Entry Name: BRD4_HUMAN

NCBI Description

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

BRD4: an atypical protein kinase of the Brd family. Contains a double bromodomain. Binds to chromatin and regulates cell cycle progression at multiple stages. Associates with chromosomes during mitosis. The cognate gene is the chromosome 19 target of translocation t(15;19)(q13;p13.1), resulting in the BRD4-NUT oncogene identified in a lethal carcinoma of young people. Binds to papillomavirus E2 protein and is required for its transforming ability. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, atypical; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; BRD family

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: nucleoplasm; transcription elongation factor complex b; condensed nuclear chromosome; nuclear chromatin; cytoplasm; chromosome; nucleus

Molecular Function: protein binding; DNA binding; p53 binding; chromatin binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; transcription, DNA-dependent; inner cell mass cell proliferation; protein amino acid phosphorylation; chromosome segregation; chromatin remodeling; G1/S-specific transcription in mitotic cell cycle; positive regulation of RNA elongation from RNA polymerase II promoter; regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus; positive regulation of DNA binding