Rat selenoprotein P, plasma, 1 (SEPP1) ELISA kit | SEPP1 elisa kit

Rat selenoprotein P, plasma, 1, SEPP1 ELISA Kit

Cat. Num. AAA944361

• Reactivity: Rat
• Synonyms: selenoprotein P; plasma; 1 (SEPP1) ELISA kit; Rat selenoprotein P; 1; SEPP1 ELISA Kit; SELP; SeP; SEPP1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat SEPP1. No significant cross-reactivity or interference between rat SEPP1 and analogues was observed.
• Sequence Length: 385

• Samples: serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 78 pg/ml - 5000 pg/ml
• Sensitivity: <19.5 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEPP1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEPP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEPP1present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEPP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEPP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 144953874
• NCBI GeneID: 29360
• NCBI Accession #: NP_001077380.1
• NCBI GenBank Nucleotide #: NM_001083911.1
• UniProt Accession #: P25236
• Molecular Weight: 43,083 Da
• NCBI Official Full Name: selenoprotein P
• NCBI Official Synonym Full Names: selenoprotein P, plasma, 1
• NCBI Official Symbol: Sepp1
• NCBI Protein Information: selenoprotein P; seP
• UniProt Protein Name: Selenoprotein P
• Protein Family: Selenoprotein
• UniProt Gene Name: Sepp1
• UniProt Synonym Gene Names: Selp; SeP
• UniProt Entry Name: SEPP1_RAT

NCBI Description

This gene encodes a selenoprotein containing multiple selenocysteine (Sec) residues encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is a secreted protein, and is unusual in that it contains 10 selenocysteine residues per polypeptide, constituting most of the selenium in plasma. It has been implicated as an extracellular antioxidant and in the transport of selenium. Transcript variants differing in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SEPP1: Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis. Belongs to the selenoprotein P family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: selenium binding

Biological Process: response to selenium ion; sexual reproduction; locomotory behavior; brain development; selenium metabolic process; growth; post-embryonic development