Rat Selenoprotein P1, Plasma (SEPP1) RTU ELISA Kit | SEPP1 rtu elisa kit

Rat Selenoprotein P1, Plasma (SEPP1) ELISA Kit

Cat. Num. AAA4502441

• Gene Names: Selenop; Sepp1
• Reactivity: Rat
• Synonyms: Selenoprotein P1; Plasma (SEPP1); Rat Selenoprotein P1; Plasma (SEPP1) ELISA Kit; SEPP; SeP; SEP-P1; SELP; SEPP1 rtu elisa kit

• Reactivity: Rat
• Sequence Length: 385

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 6.25-400ng/mL
• Sensitivity: 2.24ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEPP1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SEPP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SEPP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SEPP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SEPP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 144953874
• NCBI GeneID: 29360
• NCBI Accession #: NP_001077380.1
• NCBI GenBank Nucleotide #: NM_001083911.1
• UniProt Accession #: P25236
• Molecular Weight: 43,083 Da
• NCBI Official Full Name: selenoprotein P
• NCBI Official Synonym Full Names: selenoprotein P
• NCBI Official Symbol: Selenop
• NCBI Official Synonym Symbols: Sepp1
• NCBI Protein Information: selenoprotein P
• UniProt Protein Name: Selenoprotein P
• Protein Family: Selenoprotein
• UniProt Gene Name: Selenop
• UniProt Synonym Gene Names: SeP

NCBI Description

This gene encodes a selenoprotein that is predominantly expressed in the liver and secreted into the plasma. This selenoprotein is unique in that it contains multiple selenocysteine (Sec) residues per polypeptide (10 in rat), and accounts for most of the selenium in plasma. It has been implicated as an extracellular antioxidant, and in the transport of selenium to extra-hepatic tissues via apolipoprotein E receptor-2 (apoER2). Mice lacking this gene exhibit neurological dysfunction, suggesting its importance in normal brain function. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. The mRNA for this selenoprotein contains two SECIS elements. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2017]

Uniprot Description

Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis.