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Rabbit UBF Polyclonal Antibody | anti-UBF antibody

UBF (Phospho-Thr117) Conjugated Antibody

Gene Names
UBTF; UBF; UBF1; UBF-1; NOR-90
Reactivity
Human, Mouse, Rat
Applications
Most Applications
Synonyms
UBF; Polyclonal Antibody; UBF (Phospho-Thr117) Conjugated Antibody; 90 kDa nucleolus organizer region autoantigen; Autoantigen NOR-90; NOR 90; Nucleolar transcription factor 1; UBF 1; UBF-1; UBF1_HUMAN; Ubtf; Upstream binding factor 1; upstream binding transcription factor; RNA polymerase I; Upstream-binding factor 1; anti-UBF antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Phospho-UBF (Phospho-Thr117) antibody detects endogenous levels of UBF only when phosphorylated at Thr117
Form/Format
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
Applicable Applications for anti-UBF antibody
Most Applications
Application Notes
Suggested Dilution:
AF350 conjugated: most applications: 1:50-1:250
AF405 conjugated: most applications: 1:50-1:250
AF488 conjugated: most applications: 1:50-1:250
AF555 conjugated: most applications: 1:50-1:250
AF594 conjugated: most applications: 1:50-1:250
AF647 conjugated: most applications: 1:50-1:250
AF680 conjugated: most applications: 1:50-1:250
AF750 conjugated: most applications: 1:50-1:250
Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1:50-1:1000
Immunogen
A synthesized peptide derived from human UBF around the phosphorylation site of Thr117
Conjugation
Biotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
Excitation Emission
AF350: 346nm/442nm
Preparation and Storage
Store at 4 degree C for 6 months.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
89,406 Da
NCBI Official Full Name
nucleolar transcription factor 1 isoform b
NCBI Official Synonym Full Names
upstream binding transcription factor, RNA polymerase I
NCBI Official Symbol
UBTF
NCBI Official Synonym Symbols
UBF; UBF1; UBF-1; NOR-90
NCBI Protein Information
nucleolar transcription factor 1; autoantigen NOR-90; 90-kDa nucleolus organizer region autoantigen
UniProt Protein Name
Nucleolar transcription factor 1
UniProt Gene Name
UBTF
UniProt Synonym Gene Names
UBF; UBF1; UBF-1
UniProt Entry Name
UBF1_HUMAN

NCBI Description

This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. [provided by RefSeq, Aug 2011]

Uniprot Description

UBF: a transcription factor that recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the species-specific factor SL1. Activate RNA polymerase II-regulated, beta-catenin-responsive promoters. Interacts with TAF1, a factor involved in the transcription of cell cycle and growth regulatory genes. It binds specifically to the upstream control element. Two splice variant isoforms have been described.

Protein type: Nucleolus; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; nucleolus

Molecular Function: protein binding; DNA binding; chromatin binding

Biological Process: chromatin remodeling; regulation of transcription from RNA polymerase I promoter; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; positive regulation of transcription from RNA polymerase I promoter

Research Articles on UBF

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Product Notes

The UBF ubtf (Catalog #AAA9438436) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBF (Phospho-Thr117) Conjugated Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's UBF can be used in a range of immunoassay formats including, but not limited to, Most Applications. Suggested Dilution: AF350 conjugated: most applications: 1:50-1:250 AF405 conjugated: most applications: 1:50-1:250 AF488 conjugated: most applications: 1:50-1:250 AF555 conjugated: most applications: 1:50-1:250 AF594 conjugated: most applications: 1:50-1:250 AF647 conjugated: most applications: 1:50-1:250 AF680 conjugated: most applications: 1:50-1:250 AF750 conjugated: most applications: 1:50-1:250 Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1:50-1:1000. Researchers should empirically determine the suitability of the UBF ubtf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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