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Western Blot (WB) (Western blot analysis of VAV2 on SW480 cells lysates using anti-VAV2 antibody at 1/1, 000 dilution.)

Rabbit VAV2 Monoclonal Antibody | anti-VAV2 antibody

VAV2 Antibody

Gene Names
VAV2; VAV-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
VAV2; Monoclonal Antibody; VAV2 Antibody; Guanine nucleotide exchange factor VAV2 antibody; Oncogene VAV2 antibody; Protein vav 2 antibody; VAV 2 antibody; Vav 2 oncogene antibody; VAV-2 antibody; VAV2 antibody; VAV2 oncogene antibody; VAV2_HUMAN antibody; anti-VAV2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SC68-03
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-VAV2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:1000-5000
IHC: 1:50-1:200
ICC: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of VAV2 on SW480 cells lysates using anti-VAV2 antibody at 1/1, 000 dilution.)

Western Blot (WB) (Western blot analysis of VAV2 on SW480 cells lysates using anti-VAV2 antibody at 1/1, 000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-VAV2 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-VAV2 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VAV2 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VAV2 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining VAV2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining VAV2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining VAV2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining VAV2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining VAV2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining VAV2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-VAV2 antibody
The Vav gene was originally identified on the basis of its oncogenic activation during the course of gene transfer assays. The major translational product of the Vav proto-oncogene has been identified as a protein containing an array of structural motifs. This protein, known as Vav, Vav1 or p95Vav, contains an N-terminal helix-loop-helix domain and a leucine zipper motif similar to that of Myc family proteins that, if deleted, causes oncogenic activation. In addition, Vav contains an SH2 domain, which could indicate its role as a substrate for tyrosine kinases. Expression of Vav is limited exclusively to cells of hematopoietic origin, including those of the erythroid, lymphoid and myeloid lineages. These results suggest that Vav may represent a new type of signal transduction molecule involved in the transduction of tyrosine phosphorylation signaling into transcriptional events. Vav2 is a member of the Vav family of oncoproteins and acts as a guanosine nucleotide exchange factor (GEF) for RhoG and RhoA-like GTPases in a phosphotyrosine-dependent manner.
Product Categories/Family for anti-VAV2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
101,289 Da
NCBI Official Full Name
guanine nucleotide exchange factor VAV2 isoform 1
NCBI Official Synonym Full Names
vav 2 guanine nucleotide exchange factor
NCBI Official Symbol
VAV2
NCBI Official Synonym Symbols
VAV-2
NCBI Protein Information
guanine nucleotide exchange factor VAV2; vav 2 oncogene
UniProt Protein Name
Guanine nucleotide exchange factor VAV2
UniProt Gene Name
VAV2
UniProt Synonym Gene Names
VAV-2
UniProt Entry Name
VAV2_HUMAN

NCBI Description

VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

VAV2: a proto-oncogenic guanine nucleotide exchange factors (GEF) of the Dbl family. A GEF for the Rho family of GTP binding proteins. Expressed in most tissues.

Protein type: GEFs, Rac/Rho; GEFs; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: plasma membrane; cytosol

Molecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding; metal ion binding; guanyl-nucleotide exchange factor activity; epidermal growth factor receptor binding

Biological Process: lamellipodium biogenesis; axon guidance; platelet activation; cell migration; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of phosphoinositide 3-kinase activity; signal transduction; regulation of small GTPase mediated signal transduction; regulation of gene expression; small GTPase mediated signal transduction; regulation of blood coagulation; ephrin receptor signaling pathway; innate immune response; angiogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway

Research Articles on VAV2

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Product Notes

The VAV2 vav2 (Catalog #AAA9435786) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VAV2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's VAV2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:1000-5000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the VAV2 vav2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VAV2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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