Rabbit MC4-R Polyclonal Antibody | anti-MC4-R antibody

MC4-R (E315) Polyclonal Antibody

Cat. Num. AAA3003067

• Gene Names: MC4R; BMIQ20
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MC4-R; Polyclonal Antibody; MC4-R (E315) Polyclonal Antibody; Melanocortin receptor 4; MC4R; anti-MC4-R antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MC4-R (E315) polyclonal antibody detects endogenous levels of MC4-R protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 332

• Applicable Applications for anti-MC4-R antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human MC4-R.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Related Product Information for anti-MC4-R antibody

MC4 receptor is the fourth member of the five known melanocortin receptors (MCRs) belonging to the rhodopsin-like family of G protein-coupled receptors (GPCRs). These five MCRs form a unique family of GPCRs, marked by their unusually short coding sequence and the absence of highly conserved amino acid residues or motifs common to most GPCRs. All MCRs signal through the cyclic adenosine 3', 5'-monophosphate (cAMP) pathway. With the exception of MC2R, all MCRs are activated by both adrenocorticotropin (ACTH) and melanocyte-stimulating hormones (MSHs and mainly alpha-MSH). The MC4R receptor is expressed primarily in the brain, and unlike the other melanocortin receptors, MC4R is not expressed in adrenal cortex or in melanocytes. Genetic studies of mice and humans have established a critical role of MC4 Receptor in appetite regulation. Heterozygous mutations in MC4 Receptor account for 1-6% of severe cases of human obesity.

NCBI and Uniprot Product Information

• NCBI GI #: 119508433
• NCBI GeneID: 4160
• NCBI Accession #: NP_005903.2
• NCBI GenBank Nucleotide #: NP_005903.2
• UniProt Accession #: P32245
• Molecular Weight: ~ 37kDa
• NCBI Official Full Name: melanocortin receptor 4
• NCBI Official Synonym Full Names: melanocortin 4 receptor
• NCBI Official Symbol: MC4R
• NCBI Official Synonym Symbols: BMIQ20
• NCBI Protein Information: melanocortin receptor 4
• UniProt Protein Name: Melanocortin receptor 4
• UniProt Gene Name: MC4R
• UniProt Synonym Gene Names: MC4-R
• UniProt Entry Name: MC4R_HUMAN

NCBI Description

The protein encoded by this gene is a membrane-bound receptor and member of the melanocortin receptor family. The encoded protein interacts with adrenocorticotropic and MSH hormones and is mediated by G proteins. This is an intronless gene. Defects in this gene are a cause of autosomal dominant obesity. [provided by RefSeq, Jan 2010]

Uniprot Description

MC4R: receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. A G-protein coupled receptor that stimulates adenylate cyclase. Defects are a cause of autosomal dominant obesity

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 18q22

Cellular Component: integral to membrane; plasma membrane; nucleus

Molecular Function: neuropeptide binding; protein binding; melanocortin receptor activity; peptide hormone binding; ubiquitin protein ligase binding; melanocyte stimulating hormone receptor activity

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; diet induced thermogenesis; insulin secretion; positive regulation of cAMP biosynthetic process; energy reserve metabolic process; positive regulation of bone resorption; feeding behavior; response to insulin stimulus

Disease: Obesity