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Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TPH1 antibody. Counter stained with hematoxylin.)

Rabbit Tryptophan Hydroxylase 1 (TPH1) Monoclonal Antibody | anti-TPH1 antibody

Tryptophan Hydroxylase 1 (TPH1) Antibody

Gene Names
TPH1; TPRH; TRPH
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Tryptophan Hydroxylase 1 (TPH1); Monoclonal Antibody; Tryptophan Hydroxylase 1 (TPH1) Antibody; Indoleacetic acid 5 hydroxylase antibody; L tryptophan hydroxylase antibody; MGC119994 antibody; TPH 1 antibody; TPH antibody; TPH1 antibody; TPH1_HUMAN antibody; TPRH antibody; TRPH antibody; Tryptophan 5 hydroxylase 1 antibody; Tryptophan 5 monooxygenase 1 antibody; Tryptophan 5 monooxygenase antibody; Tryptophan 5-hydroxylase 1 antibody; Tryptophan 5-monooxygenase 1 antibody; Tryptophan hydroxylase 1 antibody; anti-TPH1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SC53-07
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-TPH1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TPH1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TPH1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-TPH1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-TPH1 antibody. Counter stained with hematoxylin.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Hela cells with TPH1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of Hela cells with TPH1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-TPH1 antibody
Phenylalanine hydroxylase (PAH), tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) comprise a small family of monooxygenases that use tetrahydropterine as a cofactor during the catabolism of aromatic L-amino acids. PAH, TH and TPH all contain catalytic domains with an amino-terminal regulatory domain and a short carboxy-terminal tetramerization domain. Each of these enzymes also contains a single ferrous iron atom, which is bound to two histidines and a glutamate and is likely to be involved in the formation of the hydroxylating intermediate. TPH is the first and rate-limiting step in the biosynthesis of serotonin in the central nervous system and melatonin in the pineal gland. Alteration of TPH function may be a key factor in the pathology of several neuropsychiatric disorders associated with serotonin, including depression, aggression, alcoholism and schizophrenia. For instance, L-DOPA, which is used as a common therapy for Parkinsons disease (PD) patients, inhibits TPH function, which subsequently, is thought to contribute to the onset of depression in PD patients.
Product Categories/Family for anti-TPH1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,985 Da
NCBI Official Full Name
tryptophan 5-hydroxylase 1
NCBI Official Synonym Full Names
tryptophan hydroxylase 1
NCBI Official Symbol
TPH1
NCBI Official Synonym Symbols
TPRH; TRPH
NCBI Protein Information
tryptophan 5-hydroxylase 1; L-tryptophan hydroxylase; tryptophan 5-monooxygenase 1; indoleacetic acid-5-hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase)
UniProt Protein Name
Tryptophan 5-hydroxylase 1
Protein Family
UniProt Gene Name
TPH1
UniProt Synonym Gene Names
TPH; TPRH; TRPH
UniProt Entry Name
TPH1_HUMAN

NCBI Description

This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009]

Uniprot Description

TPH1: an enzyme that is rate-limiting in the biosynthesis of serotonin. It catalyzes the biopterin dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Its expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. Two alternatively spliced isoforms have been described.

Protein type: Oxidoreductase; Amino Acid Metabolism - tryptophan; EC 1.14.16.4

Chromosomal Location of Human Ortholog: 11p15.3-p14

Cellular Component: neuron projection; cytosol

Molecular Function: amino acid binding; tryptophan 5-monooxygenase activity; iron ion binding

Biological Process: circadian rhythm; aromatic amino acid family metabolic process; indolalkylamine biosynthetic process; negative regulation of ossification; positive regulation of fat cell differentiation; bone remodeling; serotonin biosynthetic process

Research Articles on TPH1

Similar Products

Product Notes

The TPH1 tph1 (Catalog #AAA9435743) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Tryptophan Hydroxylase 1 (TPH1) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Tryptophan Hydroxylase 1 (TPH1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the TPH1 tph1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tryptophan Hydroxylase 1 (TPH1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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