Mouse Heparanase ELISA Kit | Hpse elisa kit

Mouse Heparanase ELISA Kit

Cat. Num. AAA9426011

• Gene Names: Hpse; Hpa; HSE1; Hpr1
• Reactivity: Mouse
• Synonyms: Heparanase; Mouse Heparanase ELISA Kit; Endo-glucoronidase; Hpse; Hpa; 3.2.1.166; Hpse elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Detection Range: 78-5000 pg/mL
• Assay Time: 3-5h
• Sample Volume: 100T
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 4 degree C

NCBI and Uniprot Product Information

• NCBI GI #: 22779887
• NCBI GeneID: 15442
• NCBI Accession #: NP_690016.1
• NCBI GenBank Nucleotide #: NM_152803.5
• UniProt Accession #: Q6YGZ1; Q8K3K3; B2RS99
• Molecular Weight: 60,066 Da
• NCBI Official Full Name: heparanase preproprotein
• NCBI Official Synonym Full Names: heparanase
• NCBI Official Symbol: Hpse
• NCBI Official Synonym Symbols: Hpa; HSE1; Hpr1
• NCBI Protein Information: heparanase
• UniProt Protein Name: Heparanase
• Protein Family: Heparanase
• UniProt Gene Name: Hpse
• UniProt Synonym Gene Names: Hpa

NCBI Description

This gene encodes an endoglucuronidase enzyme that plays an important role in tumor invasion and metastasis. The encoded preproprotein undergoes proteolytic processing to generate an active heterodimeric enzyme that cleaves the heparan sulfate proteoglycans associated with the cell surface and extracellular matrix. Mice lacking the encoded protein do not show any prominent pathological alterations under normal conditions but fail to develop albuminuria and renal damage in response to drug-induced diabetes. [provided by RefSeq, Aug 2016]

Uniprot Description

HPSE: Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up- regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis. Belongs to the glycosyl hydrolase 79 family.

Protein type: EC 3.2.1.166; Extracellular matrix; Glycan Metabolism - glycosaminoglycan degradation; Hydrolase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5|5 E4

Cellular Component: intracellular membrane-bound organelle; lipid raft; lysosome; nucleoplasm; nucleus; proteinaceous extracellular matrix

Molecular Function: heparanase activity; protein dimerization activity; syndecan binding

Biological Process: angiogenesis involved in wound healing; cell-matrix adhesion; heparan sulfate proteoglycan catabolic process; positive regulation of blood coagulation; positive regulation of hair follicle development; positive regulation of osteoblast proliferation; positive regulation of protein kinase B signaling cascade; regulation of hair follicle development; wound healing