Mouse IL-10 ELISA Kit | IL-10 elisa kit

Mouse IL-10 ELISA KIT

Cat. Num. AAA9424851

• Gene Names: IL10; CSIF; IL-10
• Reactivity: Mouse
• Synonyms: IL-10; Mouse IL-10 ELISA KIT; Mouse IL-10; IL-10 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse IL-10 Ligand

• Assay Type: Solid Phase Sandwich ELISA
• Samples: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Detection Range: 0.02-1.0 ng/mL
• Sensitivity: 7pg/mL
• Assay Time: 3 hour
• Sample Volume: 20 uL
• Detection Method: Colorimetric
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

(Representative standard curve for IL-10 ELISA. IL-10 was diluted in serial two-fold steps in Sample Diluent.)

Related Product Information for IL-10 elisa kit

Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitor, is a pleiotropic cytokine that inhibits the production of a number of cytokines (including IL-1, GM-CSF, TNF, IL-6, IL-8, IL-12 and IFN-g) by activated Th1 cells, NK cells, and monocytes/macrophages. Mouse IL-10 is produced by a wide variety of cells, including activated Th2 cells, fetal thymocytes, monocytes/macrophages, keratinocytes, B cells, and glial cells (1-3). Mouse IL-10 cDNA encodes a 178 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to generate the 160 amino acid residue mature protein (4). At the amino acid sequence level, there is approximately 73% identity between mouse and rat or mouse and human IL-10. Although human IL-10 is active on mouse cells, mouse IL-10 does not show species cross-reactivity on human cells (1). Herpes viruses, including EBV and equine herpes virus type 2, have been shown to encode viral homologues of IL-10 that exhibit some of the activities of IL-10 on both mouse and human cells (5, 6).IL-10 has been shown to inhibit macrophage cytotoxic activity and to stimulate the proliferation and differentiation of B cells, mast cells, and thymic T cells. IL-10 is a potent modulator of monocyte/macrophage function. IL-10 also enhances the release of soluble TNF receptors and inhibits the expression of surface ICAM-1 and B7 (7, 8). Finally, IL-10 has been reported to suppress the synthesis of superoxide anion (9) plus reactive oxygen intermediates (ROI), and either inhibit or facilitate nitric oxide synthesis, depending on the time of exposure to activated macrophages (10). IL-10 has marked effects on B cells. IL-10 also has documented activity on endothelial cells, where it mimics IL-4 (11), and on thymocytes (12) and mast cells, where it acts as a growth costimulator (13, 14).

NCBI and Uniprot Product Information

• NCBI GI #: 385202765
• NCBI GeneID: 397106
• Molecular Weight: 19,934 Da
• NCBI Official Full Name: IL-10, partial
• NCBI Official Symbol: IL10
• NCBI Official Synonym Symbols: CSIF; IL-10
• NCBI Protein Information: interleukin-10
• UniProt Protein Name: Interleukin-10
• UniProt Gene Name: IL10
• UniProt Synonym Gene Names: IL-10; CSIF

Uniprot Description

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.