Typical Testing Data/Standard Curve (for reference only)
(Representative standard curve for IL-5 ELISA. IL-5 was diluted in serial two-fold steps in Sample Diluent.)
Mouse IL-5 ELISA Kit | IL-5 elisa kit
Mouse IL-5 ELISA KIT
Gene Names
Il5; Il-5
Reactivity
Mouse
Synonyms
IL-5; Mouse IL-5 ELISA KIT; Mouse IL-5; IL-5 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse IL-5 Ligand
Assay Type
Solid Phase Sandwich ELISA
Samples
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Detection Range
0.02-1.0 ng/mL
Sensitivity
7pg/mL
Assay Time
3 hour
Sample Volume
20 uL
Detection Method
Colorimetric
Preparation and Storage
Store at 4 degree C
Typical Testing Data/Standard Curve (for reference only)
(Representative standard curve for IL-5 ELISA. IL-5 was diluted in serial two-fold steps in Sample Diluent.)
Typical Testing Data/Standard Curve (for reference only)
(Representative standard curve for IL-5 ELISA. IL-5 was diluted in serial two-fold steps in Sample Diluent.)
Related Product Information for IL-5 elisa kit
Mouse interleukin 5 (mIL-5), also known as T-cell replacing factor, B-cell growth factor II, eosinophil differentiation factor and eosinophil colony stimulating factor, is a pleiotropic cytokine produced primarily by T cells (1-4). It supports the proliferation and differentiation of mouse, but not human, B cells, and enhances IgM, IgG1, IgA, and IgE secretion. IL-5 chemoattracts and enhances the survival and the effector functions of mature eosinophils and synergizes with various colony stimulating factors to increase eosinophil progenitor production and eosinophil expansion. Because of its diverse effects on eosinophils, IL-5 is strongly implicated in the pathogenesis of asthma and other hypereosinophilic inflammatory conditions (4-6). Mouse IL-5 cDNA encodes a 133 amino acid (aa) residue precursor protein containing a hydrophobic signal peptide that is cleaved to yield a 113 aa residue mature protein. Native mouse IL-5 is a disulfide-linked homodimeric 40-45 kDa glycoprotein with N- and O-linked carbohydrate chains. Mature human IL-5 is approximately 70% identical at the amino acid level to mouse IL-5. Whereas mouse and human IL-5 are equally active on human cell lines, human IL-5 is much less active than mouse IL-5 in mouse cell assays (1-3). IL-5 is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
15,410 Da
NCBI Official Full Name
Interleukin-5
NCBI Official Synonym Full Names
interleukin 5
NCBI Official Symbol
Il5
NCBI Official Synonym Symbols
Il-5
NCBI Protein Information
interleukin-5
UniProt Protein Name
Interleukin-5
UniProt Gene Name
Il5
UniProt Synonym Gene Names
Il-5; IL-5; BCGF-II; TRF
Uniprot Description
IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.
Protein type: Cytokine; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11 B1.3|11 31.99 cM
Cellular Component: extracellular region; extracellular space
Molecular Function: cytokine activity; interleukin-5 receptor binding
Biological Process: cytokine and chemokine mediated signaling pathway; positive regulation of B cell proliferation; positive regulation of cell proliferation; positive regulation of eosinophil differentiation; positive regulation of immunoglobulin secretion; positive regulation of JAK-STAT cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription factor activity; positive regulation of transcription, DNA-dependent
Research Articles on IL-5
Similar Products
Product Notes
The Mouse IL-5 il5 (Catalog #AAA9424855) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9424855 ELISA Kit recognizes Mouse IL-5. It is sometimes possible for the material contained within the vial of "IL-5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
