Na (+)/H (+) exchange regulatory cofactor NHE-RF3 Recombinant Protein | NHRF3 recombinant protein

Recombinant Human Na (+)/H (+) exchange regulatory cofactor NHE-RF3

Cat. Num. AAA9421201

• Gene Names: PDZK1; CAP70; CLAMP; PDZD1; NHERF3; NHERF-3
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Na (+)/H (+) exchange regulatory cofactor NHE-RF3; Recombinant Human Na (+)/H (+) exchange regulatory cofactor NHE-RF3; CFTR-associated protein of 70 kDaNa (+)/H (+) exchanger regulatory factor 3Na/Pi cotransporter C-terminal-associated protein 1; NaPi-Cap1; PDZ domain-containing protein 1Sodium-hydrogen exchanger regulatory factor 3; NHRF3 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Full Length, 1-519aa

• Tag Info: His-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for NHRF3 recombinant protein

A scaffold protein that connects plasma mbrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated mbrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. Required for normal cell-surface expression of SCARB1. Plays a role in maintaining normal plasma cholesterol levels via its effects on SCARB1. Plays a role in the normal localization and function of the chloride-anion exchanger SLC26A6 to the plasma mbrane in the brush border of the proximal tubule of the kidney. May be involved in the regulation of proximal tubular Na+-dependent inorganic phosphate cotransport therefore playing an important role in tubule function.

NCBI and Uniprot Product Information

• NCBI GI #: 318037203
• NCBI GeneID: 5174
• NCBI Accession #: NP_001188254.1
• NCBI GenBank Nucleotide #: NM_001201325.1
• UniProt Accession #: Q5T2W1; O60450; Q5T5P6; Q9BQ41; B4DPB9; E7EU02
• Molecular Weight: 61.2kD
• NCBI Official Full Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF3 isoform 1
• NCBI Official Synonym Full Names: PDZ domain containing 1
• NCBI Official Symbol: PDZK1
• NCBI Official Synonym Symbols: CAP70; CLAMP; PDZD1; NHERF3; NHERF-3
• NCBI Protein Information: Na(+)/H(+) exchange regulatory cofactor NHE-RF3
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF3
• UniProt Gene Name: PDZK1
• UniProt Synonym Gene Names: CAP70; NHERF3; PDZD1; NHERF-3; NaPi-Cap1

NCBI Description

This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

PDZK1: A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function. Belongs to the NHER family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: brush border membrane; plasma membrane

Molecular Function: PDZ domain binding; protein binding; transporter activity

Biological Process: carnitine transport; cell proliferation; transport