Glycine oxidase Recombinant Protein | GLOX recombinant protein

Recombinant Bacillus subtilis Glycine oxidase

Cat. Num. AAA9421095

• Reactivity: BACSU
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Glycine oxidase; Recombinant Bacillus subtilis Glycine oxidase; GLOX recombinant protein

• Host: E Coli
• Reactivity: BACSU
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Full Length, 1-369aa

• Tag Info: His-SUMO-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for GLOX recombinant protein

Catalyzes the FAD-dependent oxidative deamination of various amines and D-amino acids to yield the corresponding alpha-keto acids, ammonia/amine, and hydrogen peroxide. Oxidizes sarcosine (N-methylglycine), N-ethylglycine and glycine. Can also oxidize the herbicide glyphosate (N-phosphonomethylglycine). Displays lower activities on D-alanine, D-valine, D-proline and D-methionine. Does not act on L-amino acids and other D-amino acids. Is essential for thiamine biosynthesis since the oxidation of glycine catalyzed by ThiO generates the glycine imine intermediate (dehydroglycine) required for the biosynthesis of the thiazole ring of thiamine pyrophosphate.

NCBI and Uniprot Product Information

• NCBI GI #: 16078232
• NCBI GeneID: 939377
• NCBI Accession #: NP_389049.1
• NCBI GenBank Nucleotide #: NC_000964.3
• UniProt Accession #: O31616
• Molecular Weight: 56.9kD
• NCBI Official Full Name: glycine oxidase
• NCBI Official Symbol: thiO
• NCBI Protein Information: glycine oxidase
• UniProt Protein Name: Glycine oxidase
• Protein Family: Glycine oxidase
• UniProt Gene Name: thiO
• UniProt Synonym Gene Names: GO

Uniprot Description

Catalyzes the FAD-dependent oxidative deamination of various amines and D-amino acids to yield the corresponding alpha-keto acids, ammonia/amine, and hydrogen peroxide. Oxidizes sarcosine (N-methylglycine), N-ethylglycine and glycine (PubMed:9827558, PubMed:11744710, PubMed:19864430). Can also oxidize the herbicide glyphosate (N-phosphonomethylglycine) (PubMed:19864430). Displays lower activities on D-alanine, D-valine, D-proline and D-methionine (PubMed:9827558, PubMed:11744710). Does not act on L-amino acids and other D-amino acids (PubMed:9827558). Is essential for thiamine biosynthesis since the oxidation of glycine catalyzed by ThiO generates the glycine imine intermediate (dehydroglycine) required for the biosynthesis of the thiazole ring of thiamine pyrophosphate (PubMed:12627963).