Growth/differentiation factor 5 Recombinant Protein | GDF5 recombinant protein

Recombinant mouse Growth/differentiation factor 5

Cat. Num. AAA9420263

• Gene Names: Gdf5; bp; brp; BMP-14; Cdmp-1
• Reactivity: Mouse
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Growth/differentiation factor 5; Recombinant mouse Growth/differentiation factor 5; Bone morphogenetic protein 14; BMP-14; GDF5 recombinant protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Full Length, 376-495aa

• Tag Info: His-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for GDF5 recombinant protein

Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction. Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG. Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 224922797
• NCBI GeneID: 14563
• NCBI Accession #: NP_032135.2
• NCBI GenBank Nucleotide #: NM_008109.3
• UniProt Accession #: P43027; A2ARK2
• Molecular Weight: 17.7kD
• NCBI Official Full Name: growth/differentiation factor 5 preproprotein
• NCBI Official Synonym Full Names: growth differentiation factor 5
• NCBI Official Symbol: Gdf5
• NCBI Official Synonym Symbols: bp; brp; BMP-14; Cdmp-1
• NCBI Protein Information: growth/differentiation factor 5
• UniProt Protein Name: Growth/differentiation factor 5
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: Gdf5
• UniProt Synonym Gene Names: Bmp14; Bp; Gdf-5; GDF-5; BMP-14

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates the development of numerous tissue and cell types, including cartilage, joints, brown fat, teeth, and the growth of neuronal axons and dendrites. Mice with a mutation in this gene exhibit enhanced tooth enamel formation. [provided by RefSeq, Aug 2016]

Uniprot Description

GDF5: a cytokine that is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. These cytokines are characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Binds to bone morphogenetic protein receptors (BMPRs), a family of transmembrane serine/threonine kinases. BMPRs are involved in bone and cartilage formation. Chondrogenic signaling is mediated by the high-affinity receptor BMPR1B. Defects in GDF5 are the cause of acromesomelic chondrodysplasia Grebe type (AMDG), acromesomelic chondrodysplasia Hunter-Thompson type (AMDH), brachydactyly type C (BDC), Du Pan syndrome (DPS), symphalangism proximal syndrome (SYM1), multiple synostoses syndrome type 2 (SYNS2), and brachydactyly type A2 (BDA2). Genetic variations in GDF5 are associated with susceptibility to osteoarthritis type 5 (OS5). Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2 H1|2 77.26 cM

Cellular Component: extracellular space

Molecular Function: cytokine activity; identical protein binding; protein binding; receptor binding; transforming growth factor beta receptor binding

Biological Process: cell development; chondrocyte differentiation; embryonic limb morphogenesis; forelimb morphogenesis; hindlimb morphogenesis; negative regulation of chondrocyte differentiation; negative regulation of epithelial cell proliferation; negative regulation of neuron apoptosis; positive regulation of BMP signaling pathway; positive regulation of chondrocyte differentiation; positive regulation of neuron differentiation; regulation of MAPKKK cascade; regulation of multicellular organism growth; SMAD protein nuclear translocation; transmembrane receptor protein serine/threonine kinase signaling pathway