Rabbit anti-Human Actin Polyclonal Antibody | anti-ACTG1 antibody

Actin antibody

Cat. Num. AAA9412606

• Gene Names: ACTG1; ACT; ACTG; BRWS2; DFNA20; DFNA26; HEL-176
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: Actin; Polyclonal Antibody; Actin antibody; anti-ACTG1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 375

• Applicable Applications for anti-ACTG1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:3000; Immunohistochemistry: 1:100-1:250
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 119 of human ACTG1
• Target Name: Actin
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE Primary antibody diluted at 1: 1000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Spleen, using gamma Actin antibody(10 ug/ml).)

Related Product Information for anti-ACTG1 antibody

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells. [provided by RefSeq]

Product Categories/Family for anti-ACTG1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4501887
• NCBI GeneID: 71
• NCBI Accession #: NP_001605
• NCBI GenBank Nucleotide #: NM_001614.3
• Molecular Weight: 41,793 Da
• NCBI Official Full Name: actin, cytoplasmic 2
• NCBI Official Synonym Full Names: actin gamma 1
• NCBI Official Symbol: ACTG1
• NCBI Official Synonym Symbols: ACT; ACTG; BRWS2; DFNA20; DFNA26; HEL-176
• NCBI Protein Information: actin, cytoplasmic 2
• UniProt Protein Name: Actin, cytoplasmic 2
• Protein Family: Actin
• UniProt Gene Name: ACTG1
• UniProt Synonym Gene Names: ACTG
• UniProt Entry Name: ACTG_HUMAN

NCBI Description

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]

Uniprot Description

ACTG1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: filamentous actin; extracellular space; cytoskeleton; myofibril; membrane; plasma membrane; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; ATP binding

Biological Process: intercellular junction assembly and maintenance; axon guidance; retinal homeostasis; ephrin receptor signaling pathway; innate immune response; sarcomere organization; vascular endothelial growth factor receptor signaling pathway; cell motility

Disease: Baraitser-winter Syndrome 2; Deafness, Autosomal Dominant 20