Immunohistochemistry (IHC)
(MBS9603266 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit CISD1 Polyclonal Antibody | anti-CISD1 antibody
CISD1 Antibody
Gene Names
CISD1; ZCD1; MDS029; C10orf70; mitoNEET
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CISD1; Polyclonal Antibody; CISD1 Antibody; AU043990; AW743335; C10orf70; CDGSH iron sulfur domain 1; CDGSH iron-sulfur domain-containing protein 1; CISD 1; CISD1_HUMAN; D10Ertd214e; MDS029; MGC14684; MitoNEET; RGD1309529; ZCD1; Zinc finger CDGSH type domain 1; anti-CISD1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Zebrafish
Clonality
Polyclonal
Isotype
IgG
Specificity
CISD1 antibody detects endogenous levels of CISD1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
108
Applicable Applications for anti-CISD1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human CISD1.
Subcellular Location
Mitochondrion Outer Membrane
Tissue Specificity
Expression is reduced in cells derived from cystic fibrosis patients.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Similarity
Pig (88%), Zebrafish (100%), Bovine (88%), Horse (88%), Sheep (88%), Rabbit (100%), Chicken (88%), Xenopus (88%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603266 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603266 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of HepG2 cells, using CISD1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of HepG2 cells, using CISD1 antibody.)
Related Product Information for anti-CISD1 antibody
Function: Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions.
Subunit Structure: Homodimer.
Post-translational Modifications: Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.
Similarity: Belongs to the CISD protein family.
Subunit Structure: Homodimer.
Post-translational Modifications: Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.
Similarity: Belongs to the CISD protein family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 14-17 kDa
Predicted: 13 kDa
Predicted: 13 kDa
NCBI Official Full Name
CDGSH iron-sulfur domain-containing protein 1
NCBI Official Synonym Full Names
CDGSH iron sulfur domain 1
NCBI Official Symbol
CISD1
NCBI Official Synonym Symbols
ZCD1; MDS029; C10orf70; mitoNEET
NCBI Protein Information
CDGSH iron-sulfur domain-containing protein 1
UniProt Protein Name
CDGSH iron-sulfur domain-containing protein 1
Protein Family
UniProt Gene Name
CISD1
UniProt Synonym Gene Names
C10orf70; ZCD1
NCBI Description
This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria and is thought to play a role in regulation of oxidation. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2. [provided by RefSeq, Feb 2012]
Uniprot Description
Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (). May be involved in Fe-S cluster shuttling and/or in redox reactions.
Research Articles on CISD1
Similar Products
Product Notes
The CISD1 cisd1 (Catalog #AAA9603266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CISD1 Antibody reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's CISD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the CISD1 cisd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CISD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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