Mouse cartilage oligomeric matrix protein ELISA Kit | COMP elisa kit

Mouse Cartilage oligomeric matrix protein, COMP ELISA Kit

Cat. Num. AAA941188

• Gene Names: Comp; TSP5
• Reactivity: Mouse
• Synonyms: cartilage oligomeric matrix protein; Mouse Cartilage oligomeric matrix protein; COMP ELISA Kit; Mouse Cartilage oligomeric matrix protein (COMP) ELISA kit; EDM1; EPD1; MED; MGC131819; MGC149768; PSACH; THBS5; cartilage oligomeric matrix protein (pseudoachondroplasia; epiphyseal dysplasia 1; multiple) ; cartilage oligomeric matrix protein (pseudoachondropl; COMP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse COMP. No significant cross-reactivity or interference between Mouse COMP and analogues was observed.
• Sequence Length: 755

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 6.25 ng/ml-400 ng/ml
• Sensitivity: 1.56 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Apoptosis/Autophagy
• Protein Biological Process 3: Apoptosis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COMP elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COMP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COMP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COMP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COMP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 162287140
• NCBI GeneID: 12845
• NCBI Accession #: NP_057894.2
• NCBI GenBank Nucleotide #: NM_016685.2
• UniProt Accession #: Q9R0G6; G3X8Q4
• Molecular Weight: 82,342 Da
• NCBI Official Full Name: cartilage oligomeric matrix protein
• NCBI Official Synonym Full Names: cartilage oligomeric matrix protein
• NCBI Official Symbol: Comp
• NCBI Official Synonym Symbols: TSP5
• NCBI Protein Information: cartilage oligomeric matrix protein; thrombospondin-5
• UniProt Protein Name: Cartilage oligomeric matrix protein
• Protein Family: Cartilage oligomeric matrix protein
• UniProt Gene Name: Comp
• UniProt Synonym Gene Names: COMP
• UniProt Entry Name: COMP_MOUSE

Uniprot Description

COMP: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1). EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COMP are the cause of pseudoachondroplasia (PSACH). PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. Belongs to the thrombospondin family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: vitamin D binding; heparin binding; heparan sulfate proteoglycan binding; collagen binding; protein binding; protease binding; extracellular matrix structural constituent; fibronectin binding; calcium ion binding

Biological Process: limb development; extracellular matrix organization and biogenesis; apoptosis; cell adhesion; negative regulation of apoptosis