Rabbit TF2H2 Polyclonal Antibody | anti-TF2H2 antibody

TF2H2 Antibody

Cat. Num. AAA9411245

• Gene Names: GTF2H2; p44; BTF2; TFIIH; BTF2P44; T-BTF2P44
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: TF2H2; Polyclonal Antibody; TF2H2 Antibody; TFIIH basal transcription factor complex p44 subunit; Basic transcription factor 2 44 kDa subunit; BTF2-p44; General transcription factor IIH polypeptide 2; GTF2H2; anti-TF2H2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total TF2H2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 395

• Applicable Applications for anti-TF2H2 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:3000
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from N-terminal of human TF2H2.
• Target Name: TF2H2
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from COLO205 cells, using TF2H2 antibody.)

Related Product Information for anti-TF2H2 antibody

Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N-terminus interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter.Humbert S., EMBO J. 13:2393-2398(1994).The MGC Project Team, Genome Res. 14:2121-2127(2004).van der Steege G., Eur. J. Hum. Genet. 3:87-95(1995).

Product Categories/Family for anti-TF2H2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 6681762
• NCBI GeneID: 2966
• NCBI Accession #: NP_001506.1
• NCBI GenBank Nucleotide #: NM_001515.3
• UniProt Accession #: Q13888; Q15570; Q15571; Q9BS41
• Molecular Weight: 44,419 Da
• NCBI Official Full Name: general transcription factor IIH subunit 2
• NCBI Official Synonym Full Names: general transcription factor IIH, polypeptide 2, 44kDa
• NCBI Official Symbol: GTF2H2
• NCBI Official Synonym Symbols: p44; BTF2; TFIIH; BTF2P44; T-BTF2P44
• NCBI Protein Information: general transcription factor IIH subunit 2
• UniProt Protein Name: General transcription factor IIH subunit 2
• UniProt Gene Name: GTF2H2
• UniProt Synonym Gene Names: BTF2P44; BTF2 p44
• UniProt Entry Name: TF2H2_HUMAN

NCBI Description

This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This gene is within the telomeric copy of the duplication. Deletion of this gene sometimes accompanies deletion of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients but it is unclear if deletion of this gene contributes to the SMA phenotype. This gene encodes the 44 kDa subunit of RNA polymerase II transcription initiation factor IIH which is involved in basal transcription and nucleotide excision repair. Transcript variants for this gene have been described, but their full length nature has not been determined. A second copy of this gene within the centromeric copy of the duplication has been described in the literature. It is reported to be different by either two or four base pairs; however, no sequence data is currently available for the centromeric copy of the gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N-terminus interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter.