Rabbit anti-Crab Hsp70 Polyclonal Antibody | anti-HSP70 antibody

Hsp70 (Crab) Antibody: ATTO 594

Cat. Num. AAA805446

• Reactivity: Crab
• Applications: Western Blot
• Synonyms: Hsp70; Polyclonal Antibody; Hsp70 (Crab) Antibody: ATTO 594; Hsp70 1; Hsp70 2; Hsp70.1; Hsp72; HSPA1; HSPA1A; HSPA1B; anti-HSP70 antibody

• Host: Rabbit
• Reactivity: Crab
• Clonality: Polyclonal
• Form/Format: Protein A Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 650

• Applicable Applications for anti-HSP70 antibody: Western Blot (WB)
• Application Notes: 1:1000 (WB)
• Conjugate: ATTO 594
• Storage Buffer: 1X PBS pH7.4, 0.09% NaN3 and 50% glycerol.
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis of Crab Cell Lysate showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody (SPC-318). Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (SPC-318) at 1:1000.)

Related Product Information for anti-HSP70 antibody

Background Info: Detects a 70kDa protein corresponding to the molecular mass of inducible hsp70 on SDS PAGE immunoblots.

Scientific Background: Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1, 2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP-binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Product Categories/Family for anti-HSP70 antibody

Chaperones; Heat Shock; Trafficking

References

1. Welch W.J. and Suhan J.P. (1986) J.Cell Biol. 103: 2035-2050. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Hung T.H., et al. (2001) Am J Pathol. 159: 1031-1043. 8. Locke M. (2000) Cell Stress & Chaperones 5: 45-51. 9. Ianaro A., et al. (2001) FEBS Lett. 508: 61-66. 10. Trentin G.A. et al. (2001) J Biol Chem. 276: 13087-13095.

NCBI and Uniprot Product Information

• NCBI GI #: 418206099
• UniProt Accession #: B3VKG9
• Molecular Weight: 71,231 Da
• NCBI Official Full Name: heat shock protein 70
• UniProt Protein Name: Heat shock protein 70
• Protein Family: HSP70 co-chaperone
• UniProt Gene Name: HSP70
• UniProt Synonym Gene Names: hsp70
• UniProt Entry Name: B3VKG9_SCYPA