Rabbit Hax1a Polyclonal Antibody | anti-Hax1a antibody

Hax1a Antibody

Cat. Num. AAA9411115

• Gene Names: HAX1; SCN3; HS1BP1; HCLSBP1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column
• Synonyms: Hax1a; Polyclonal Antibody; Hax1a Antibody; HS-1 associated protein X-1; HCLS1 associated protein X-1; HCLSBP1; HS1BP1; anti-Hax1a antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: At least four isoforms of Hax1 are known to exist. This antibody is expected to recognize the longest isoform (Hax1a) as well as the shortest.
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 279

• Applicable Applications for anti-Hax1a antibody: ELISA (EIA), Western Blot (WB)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 15 amino acid peptide near the amino terminus of human Hax1a.
• Target Name: Hax1a
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Hax1a in human brain tissue lysate with Hax1a antibody at (A) 1 and (B) 2 ug/mL.)

Related Product Information for anti-Hax1a antibody

The HS-1 associated protein X-1 (Hax1) was initially identified in a yeast two-hybrid assay on the basis of its ability to bind to the hemapoietic cell-specific protein 1 (HS-1). Hax1 possesses anti-apoptotic activity and is structurally related to Bcl-2 family members, including the presence of BH1- and BH2-like domains. It has recently been shown to interact with HIV viral protein R (Vpr), a protein required for viral pathogenesis of HIV and linked to T-cell apoptosis through activation of caspases 3 and 9. Other studies indicate that Hax1-mediated processing of HtrA2 (also known as Omi) by the mitochondrial protease PARL allows survival of lymphocytes and neurons when cytokines are limiting.

Product Categories/Family for anti-Hax1a antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 13435356
• NCBI GeneID: 10456
• NCBI Accession #: NP_006109
• NCBI GenBank Nucleotide #: NM_006118.3
• Molecular Weight: 21,801 Da
• NCBI Official Full Name: HCLS1-associated protein X-1 isoform a
• NCBI Official Synonym Full Names: HCLS1 associated protein X-1
• NCBI Official Symbol: HAX1
• NCBI Official Synonym Symbols: SCN3; HS1BP1; HCLSBP1
• NCBI Protein Information: HCLS1-associated protein X-1
• UniProt Protein Name: HCLS1-associated protein X-1
• UniProt Gene Name: HAX1
• UniProt Synonym Gene Names: HS1BP1; HAX-1; HSP1BP-1
• UniProt Entry Name: HAX1_HUMAN

NCBI Description

The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HAX1: Promotes cell survival. Potentiates GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. May regulate intracellular calcium pools. Defects in HAX1 are the cause of neutropenia severe congenital autosomal recessive type 3 (SCN3); also known as Kostmann disease. A disorder of hematopoiesis characterized by maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Some patients affected by severe congenital neutropenia type 3 have neurological manifestations such as psychomotor retardation and seizures. The clinical phenotype due to HAX1 deficiency appears to depend on the localization of the mutations and their influence on the transcript variants. Mutations affecting exclusively isoform 1 are associated with isolated congenital neutropenia, whereas mutations affecting both isoform 1 and isoform 5 are associated with additional neurologic symptoms. Belongs to the HAX1 family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Mitochondrial; Vesicle; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: transcription factor complex; nuclear membrane; mitochondrion; sarcoplasmic reticulum; clathrin-coated vesicle; endoplasmic reticulum; lamellipodium; apical plasma membrane; nuclear envelope; actin cytoskeleton

Molecular Function: protein domain specific binding; protein binding; protein N-terminus binding; interleukin-1 binding

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; regulation of apoptosis; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; regulation of actin filament polymerization; positive regulation of granulocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation

Disease: Neutropenia, Severe Congenital, 3, Autosomal Recessive