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Testing Data (Sample(30 ug of whole cell lysate)A: 293T B: A4317.5% SDS PAGEPrimary antibody diluted at 1: 1500)

Rabbit anti-Human DNAJC7 Polyclonal Antibody | anti-DNAJC7 antibody

DNAJC7 antibody

Gene Names
DNAJC7; DJ11; DJC7; TPR2; TTC2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified by antigen-affinity chromatography.
Synonyms
DNAJC7; Polyclonal Antibody; DNAJC7 antibody; DJ11; TPR2; TTC2; DANJC7; anti-DNAJC7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Purified by antigen-affinity chromatography.
Form/Format
Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
Sequence Length
494
Applicable Applications for anti-DNAJC7 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
Immunogen Type
Recombinant protein
Immunogen Description
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 151 and 443 of DNAJC7
Target Name
DNAJC7
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample(30 ug of whole cell lysate)A: 293T B: A4317.5% SDS PAGEPrimary antibody diluted at 1: 1500)

Testing Data (Sample(30 ug of whole cell lysate)A: 293T B: A4317.5% SDS PAGEPrimary antibody diluted at 1: 1500)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DNAJC7 antibody at 1: 100 dilution.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DNAJC7 antibody at 1: 100 dilution.)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNAJC7 antibody at 1: 200 dilution.)

Immunofluorescence (IF) (Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNAJC7 antibody at 1: 200 dilution.)
Related Product Information for anti-DNAJC7 antibody
DNAJC7 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM]
Product Categories/Family for anti-DNAJC7 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,097 Da
NCBI Official Full Name
dnaJ homolog subfamily C member 7 isoform 1
NCBI Official Synonym Full Names
DnaJ (Hsp40) homolog, subfamily C, member 7
NCBI Official Symbol
DNAJC7
NCBI Official Synonym Symbols
DJ11; DJC7; TPR2; TTC2
NCBI Protein Information
dnaJ homolog subfamily C member 7
UniProt Protein Name
DnaJ homolog subfamily C member 7
Protein Family
UniProt Gene Name
DNAJC7
UniProt Synonym Gene Names
TPR2; TTC2; TPR repeat protein 2
UniProt Entry Name
DNJC7_HUMAN

NCBI Description

This gene encodes a member of the DNAJ heat shock protein 40 family of proteins that is characterized by two N-terminal tetratricopeptide repeat domains and a C-terminal DNAJ domain. This protein binds the chaperone proteins heat shock proteins 70 and 90 in an ATP-dependent manner and may function as a co-chaperone. Pseudogenes of this gene are found on chromosomes 1 and 6. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009]

Uniprot Description

DNAJC7: a co-chaperone protein that binds both Hsp70 and Hsp90 through its TPR domains. Its DnaJ domain stimulates ATP hydrolysis and polypeptide binding by Hsp70. Interacts with the proapoptotic and cell-cycle checkpoint protein Rad9. Rad9 transiently dissociates from Tpr2 following heat-shock or UV treatments.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: nucleoplasm; cytoskeleton; membrane; cytoplasm

Molecular Function: protein binding; heat shock protein binding

Biological Process: protein folding

Research Articles on DNAJC7

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Product Notes

The DNAJC7 dnajc7 (Catalog #AAA9410671) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNAJC7 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJC7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500-1:3000 Immunohistochemistry: 1:100-1:250 Immunofluorescence: 1:100-1:200. Researchers should empirically determine the suitability of the DNAJC7 dnajc7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJC7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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