Rabbit PAX3 Polyclonal Antibody | anti-PAX3 antibody

PAX3 Antibody

Cat. Num. AAA9409499

• Gene Names: PAX3; WS1; WS3; CDHS; HUP2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: PAX3; Polyclonal Antibody; PAX3 Antibody; CDHS; HUP2; WS1; WS3; anti-PAX3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PAX3 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 215

• Applicable Applications for anti-PAX3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human PAX3.
• Target Name: PAX3
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PAX3 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung cancer using PAX3 antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat liver using PAX3 antibody at dilution of 1:200 (400x lens). I)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat lung using PAX3 antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cell using PAX3 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-PAX3 antibody

Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2).

Product Categories/Family for anti-PAX3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 6654638
• NCBI GeneID: 5077
• NCBI Accession #: NP_000429.2
• NCBI GenBank Nucleotide #: NM_000438.5
• UniProt Accession #: P23760; Q16448; Q494Z3; Q494Z4; Q53T90; Q6GSJ9; Q86UQ2; Q86UQ3; G5E9C1
• Molecular Weight: 55,975 Da
• NCBI Official Full Name: paired box protein Pax-3 isoform PAX3a
• NCBI Official Synonym Full Names: paired box 3
• NCBI Official Symbol: PAX3
• NCBI Official Synonym Symbols: WS1; WS3; CDHS; HUP2
• NCBI Protein Information: paired box protein Pax-3
• UniProt Protein Name: Paired box protein Pax-3
• Protein Family: Paired box protein
• UniProt Gene Name: PAX3
• UniProt Synonym Gene Names: HUP2
• UniProt Entry Name: PAX3_HUMAN

NCBI Description

This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq, Jul 2008]

Uniprot Description

PAX3: Probable transcription factor associated with development of alveolar rhabdomyosarcoma. Can bind to DNA as a heterodimer with PAX7. Interacts with DAXX. Belongs to the paired homeobox family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; sequence-specific DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; muscle development; apoptosis; positive regulation of transcription, DNA-dependent; heart development; pigmentation during development; neuron fate commitment; negative regulation of transcription from RNA polymerase II promoter; cell proliferation; organ morphogenesis; sensory perception of sound; positive regulation of cell proliferation; neural tube closure; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of somitogenesis; neural crest cell migration

Disease: Waardenburg Syndrome, Type 3; Craniofacial-deafness-hand Syndrome; Rhabdomyosarcoma 2; Waardenburg Syndrome, Type 1