Testing Data
(Western blot analysis of extracts of A431cell lines, using PAX3 antibody.)
Rabbit PAX3 Antibody | anti-PAX3 antibody
PAX3 Antibody
Gene Names
PAX3; WS1; WS3; CDHS; HUP2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
PAX3; PAX3 Antibody; CDHS; HUP2; WS1; WS3; Paired box protein Pax-3; HuP2; anti-PAX3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
215
Applicable Applications for anti-PAX3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein of human PAX3
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Testing Data
(Western blot analysis of extracts of A431cell lines, using PAX3 antibody.)
Testing Data
(Western blot analysis of extracts of A431cell lines, using PAX3 antibody.)
Related Product Information for anti-PAX3 antibody
Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2).
Product Categories/Family for anti-PAX3 antibody
References
1. Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14. 2. Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62. 3. Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94. 4. Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50kDa
NCBI Official Full Name
paired box protein Pax-3 isoform PAX3a
NCBI Official Synonym Full Names
paired box 3
NCBI Official Symbol
PAX3
NCBI Official Synonym Symbols
WS1; WS3; CDHS; HUP2
NCBI Protein Information
paired box protein Pax-3
UniProt Protein Name
Paired box protein Pax-3
Protein Family
UniProt Gene Name
PAX3
UniProt Synonym Gene Names
HUP2
NCBI Description
This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq, Jul 2008]
Uniprot Description
Transcription factor that may regulate cell proliferation, migration and apoptosis. Involved in neural development and myogenesis. Transcriptional activator of MITF, acting synergistically with SOX10 (PubMed:21965087).
Research Articles on PAX3
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Product Notes
The PAX3 pax3 (Catalog #AAA8501081) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAX3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PAX3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the PAX3 pax3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAX3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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