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Testing Data (Gel: 10%SDS-PAGE Lysate: 40 ug Mouse heart tissue lysate Primary antibody: 1/400 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 second)

Rabbit RAB18 Polyclonal Antibody | anti-RAB18 antibody

RAB18 Antibody

Gene Names
RAB18; WARBM3; RAB18LI1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Synonyms
RAB18; Polyclonal Antibody; RAB18 Antibody; Ras-related protein Rab-18; WARBM3; RAB18LI1; anti-RAB18 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total RAB18 protein.
Form/Format
Supplied at 1.9mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
Sequence Length
206
Applicable Applications for anti-RAB18 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
ELISA: 1:2000-1:10000
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:200-1:500
Immunogen Type
Peptide-KLH
Immunogen Description
Synthetic peptide corresponding to a region derived from 128-141 amino acids of Human Ras-related protein Rab-18
Target Name
RAB18
Preparation and Storage
Store at -20 degree C/1 year

Testing Data

(Gel: 10%SDS-PAGE Lysate: 40 ug Mouse heart tissue lysate Primary antibody: 1/400 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 second)

Testing Data (Gel: 10%SDS-PAGE Lysate: 40 ug Mouse heart tissue lysate Primary antibody: 1/400 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 second)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 31143(RAB18 Antibody) at dilution 1/300, on the right is treated with the synthetic peptide.)

Immunohistochemistry (IHC) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 31143(RAB18 Antibody) at dilution 1/300, on the right is treated with the synthetic peptide.)
Related Product Information for anti-RAB18 antibody
The protein encoded by this gene is a member of a family of Ras-related small GTPases that regulate membrane trafficking in organelles and transport vesicles. Knockdown studies is zebrafish suggest that this protein may have a role in eye and brain development. Mutations in this gene are associated with Warburg micro syndrome type 3. Alternatively spliced transcript variants have been found for this gene.
Product Categories/Family for anti-RAB18 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,508 Da
NCBI Official Full Name
ras-related protein Rab-18 isoform 1
NCBI Official Synonym Full Names
RAB18, member RAS oncogene family
NCBI Official Symbol
RAB18
NCBI Official Synonym Symbols
WARBM3; RAB18LI1
NCBI Protein Information
ras-related protein Rab-18
UniProt Protein Name
Ras-related protein Rab-18
Protein Family
UniProt Gene Name
RAB18
UniProt Entry Name
RAB18_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of Ras-related small GTPases that regulate membrane trafficking in organelles and transport vesicles. Knockdown studies is zebrafish suggest that this protein may have a role in eye and brain development. Mutations in this gene are associated with Warburg micro syndrome type 3. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

RAB18: a small G protein that plays a role in apical endocytosis/recycling. May be involved in transport between the plasma membrane and early endosomes.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 10p12.1

Cellular Component: Golgi apparatus; plasma membrane; intracellular

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: intracellular protein transport; eye development; metabolic process; small GTPase mediated signal transduction; brain development; Rab protein signal transduction

Disease: Warburg Micro Syndrome 3

Research Articles on RAB18

Similar Products

Product Notes

The RAB18 rab18 (Catalog #AAA9409193) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB18 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAB18 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). ELISA: 1:2000-1:10000 Western blotting: 1:500-1:2000 Immunohistochemistry: 1:200-1:500. Researchers should empirically determine the suitability of the RAB18 rab18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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