Rabbit anti-Human HP Polyclonal Antibody | anti-HP antibody

HP Antibody

Cat. Num. AAA9408914

• Gene Names: HP; BP; HPA1S; HP2ALPHA2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Synonyms: HP; Polyclonal Antibody; HP Antibody; haptoglobin; BP; HPA1S; HP2ALPHA2; anti-HP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total HP protein.
• Form/Format: Supplied at 0.8mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 1365

• Applicable Applications for anti-HP antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1000-1:5000; Western blotting: 1:200-1:1000; Immunohistochemistry: 1:25-1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Fusion protein corresponding to a region derived from 162-406 amino acids of human haptoglobin
• Target Name: HP
• Preparation and Storage: Store at -20 degree C/1 year

Testing Data

(Gel: 10%SDS-PAGE Lysate: 30 ug Human brain gliomas tissue lysate Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 31083 (HP Antibody) at dilution 1/25, on the right is treated with the fusion protein.)

Related Product Information for anti-HP antibody

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-HP antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 111307729
• NCBI GeneID: 3240
• Molecular Weight: 38,452 Da
• NCBI Official Full Name: Homo sapiens haptoglobin, mRNA
• NCBI Official Synonym Full Names: haptoglobin
• NCBI Official Symbol: HP
• NCBI Official Synonym Symbols: BP; HPA1S; HP2ALPHA2
• NCBI Protein Information: haptoglobin
• UniProt Protein Name: Haptoglobin
• Protein Family: HPr-like protein
• UniProt Gene Name: HP
• UniProt Entry Name: HPT_HUMAN

NCBI Description

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

Uniprot Description

HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: extracellular space; extracellular region

Molecular Function: antioxidant activity; protein binding; hemoglobin binding; catalytic activity

Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; immune system process; metabolic process; negative regulation of oxidoreductase activity; defense response to bacterium; acute-phase response; defense response

Disease: Anhaptoglobinemia