Rabbit anti-Human, Mouse CARD10 Polyclonal Antibody | anti-CARD10 antibody

CARD10 Antibody

Cat. Num. AAA9408148

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: immunoaffinity purified
• Synonyms: CARD10; Polyclonal Antibody; CARD10 Antibody; Caspase recuitment domain family 10; CARMA3; Bimp1; anti-CARD10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: immunoaffinity purified
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 1032

• Applicable Applications for anti-CARD10 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human CARD10.
• Target Name: CARD10
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CARD10 expression in EL4 cell lysate with CARD10 antibody at (A) 5 and (B) 10 ug /ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of CARD10 in EL4 cells with CARD10 antibody at 10 ug/mL.)

Related Product Information for anti-CARD10 antibody

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD10. This protein belongs to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF-kB. CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the IkK complex, demonstrating its importance in the regulation of NF-kB transcription factor activation.

Product Categories/Family for anti-CARD10 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 13488607
• Molecular Weight: 82,541 Da
• NCBI Official Full Name: caspase recruitment domain protein 10
• UniProt Protein Name: Caspase recruitment domain-containing protein 10
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD10
• UniProt Synonym Gene Names: CARMA3; Carma 3
• UniProt Entry Name: CAR10_HUMAN

Uniprot Description

CARD10: a protein containing a caspase recruitment domain (CARD) that functions as an activator of BCL10 and NF-kappaB signaling. CARD is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. CARD proteins function as scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. The CARD domain of CARD14 associates specifically with the CARD domains of CARD10 and BCL10, a signaling protein that activates NF-kB through the IkappaB kinase complex in response to upstream stimuli. When expressed in cells, CARD14 activates NF-kappaB and induces the phosphorylation of BCL10. A subgroup of primary lymphomas of the central nervous system have higher levels of CARD14 expression.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: CBM complex; cytoplasm

Molecular Function: protein binding; receptor signaling complex scaffold activity

Biological Process: regulation of apoptosis; activation of NF-kappaB-inducing kinase; protein complex assembly