Rabbit PKLR Polyclonal Antibody | anti-PKLR antibody

PKLR antibody

Cat. Num. AAA9407293

• Gene Names: PKLR; PK1; PKL; PKR; RPK; PKRL
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: PKLR; Polyclonal Antibody; PKLR antibody; PK1; PKL; PKR; RPK; PKRL; anti-PKLR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PKLR antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 574

• Applicable Applications for anti-PKLR antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500 - 1:2000
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human PKLR.
• Target Name: PKLR
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of Raji cell line, using PKLR antibody.)

Related Product Information for anti-PKLR antibody

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-PKLR antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 10835121
• NCBI GeneID: 5313
• NCBI Accession #: NP_000289.1
• NCBI GenBank Nucleotide #: NM_000298.5
• UniProt Accession #: P30613; O75758; P11973
• Molecular Weight: 58,494 Da
• NCBI Official Full Name: pyruvate kinase PKLR isoform 1
• NCBI Official Synonym Full Names: pyruvate kinase, liver and RBC
• NCBI Official Symbol: PKLR
• NCBI Official Synonym Symbols: PK1; PKL; PKR; RPK; PKRL
• NCBI Protein Information: pyruvate kinase PKLR
• UniProt Protein Name: Pyruvate kinase PKLR
• Protein Family: Pyruvate kinase
• UniProt Gene Name: PKLR
• UniProt Synonym Gene Names: PK1; PKL
• UniProt Entry Name: KPYR_HUMAN

NCBI Description

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PKLR: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms have been described: the R- and L-types.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; EC 2.7.1.40; Carbohydrate Metabolism - pyruvate; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytosol

Molecular Function: potassium ion binding; magnesium ion binding; pyruvate kinase activity; ATP binding

Biological Process: pyruvate biosynthetic process; glycolysis; response to cAMP; response to lithium ion; positive regulation of cellular metabolic process; glucose metabolic process; pathogenesis; response to other organism; endocrine pancreas development; response to ATP; cellular response to insulin stimulus; response to heat; ATP biosynthetic process; carbohydrate metabolic process; response to hypoxia; energy reserve metabolic process; response to glucose stimulus; phosphorylation; response to nutrient

Disease: Adenosine Triphosphate, Elevated, Of Erythrocytes; Pyruvate Kinase Deficiency Of Red Cells