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Testing Data (Sample(30 ug whole cell lysate)A: 293T12% SDS PAGEPrimary antibody diluted at 1: 1000)

Rabbit anti-Human Peflin Polyclonal Antibody | anti-PEF1 antibody

Peflin antibody

Gene Names
PEF1; ABP32; PEF1A
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified by antigen-affinity chromatography.
Synonyms
Peflin; Polyclonal Antibody; Peflin antibody; anti-PEF1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Purified by antigen-affinity chromatography.
Form/Format
Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
Sequence Length
284
Applicable Applications for anti-PEF1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
Immunogen Type
Recombinant protein
Immunogen Description
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 77 and 265 of Peflin
Target Name
Peflin
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample(30 ug whole cell lysate)A: 293T12% SDS PAGEPrimary antibody diluted at 1: 1000)

Testing Data (Sample(30 ug whole cell lysate)A: 293T12% SDS PAGEPrimary antibody diluted at 1: 1000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using PEF1 antibody at 1: 100 dilution.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using PEF1 antibody at 1: 100 dilution.)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Peflin antibody at 1: 200 dilution.)

Immunofluorescence (IF) (Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Peflin antibody at 1: 200 dilution.)
Related Product Information for anti-PEF1 antibody
PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit (CAPNS1; MIM 114170), sorcin (SRI; MIM 182520), grancalcin (GCA; MIM 607030), and ALG2 (PDCD6; MIM 601057) (Kitaura et al., 2001 [PubMed 11278427]).[supplied by OMIM]
Product Categories/Family for anti-PEF1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,381 Da
NCBI Official Full Name
peflin
NCBI Official Synonym Full Names
penta-EF-hand domain containing 1
NCBI Official Symbol
PEF1
NCBI Official Synonym Symbols
ABP32; PEF1A
NCBI Protein Information
peflin
UniProt Protein Name
Peflin
Protein Family
UniProt Gene Name
PEF1
UniProt Synonym Gene Names
ABP32
UniProt Entry Name
PEF1_HUMAN

NCBI Description

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.[provided by RefSeq, May 2010]

Uniprot Description

PEF1: Heterodimer; heterodimerizes with PDCD6/ALG2. Dissociates from PDCD6/ALG2 in presence of Ca(2+).

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: membrane; cytoplasm

Molecular Function: protein dimerization activity; protein binding; protein heterodimerization activity; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding

Biological Process: proteolysis; response to calcium ion

Research Articles on PEF1

Similar Products

Product Notes

The PEF1 pef1 (Catalog #AAA9406821) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Peflin antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Peflin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500-1:3000 Immunohistochemistry: 1:100-1:250 Immunofluorescence: 1:100-1:200. Researchers should empirically determine the suitability of the PEF1 pef1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peflin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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