Western Blot (WB)
(Western blot detection of PEF1 in K562,Rat Brain,C6,3T3,Hela cell lysates using PEF1 Rabbit mAb(1:1000 diluted). Predicted band size:30kDa. Observed band size:30kDa.)
Rabbit PEF1 Monoclonal Antibody | anti-PEF1 antibody
PEF1 Rabbit mAb
Gene Names
PEF1; ABP32; PEF1A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Affinity purified
Synonyms
PEF1; Monoclonal Antibody; PEF1 Rabbit mAb; Peflin; ABP32; PEF1A; anti-PEF1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
R09-7F3
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-PEF1 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:1000-1:5000
FC/FACS: 1:20
IP: 1:20-1:50
FC/FACS: 1:20
IP: 1:20-1:50
Immunogen
A synthetic peptide of human PEF1
Conjugation
Unconjugated
Modification
Unmodification
Subcellular Location
Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot detection of PEF1 in K562,Rat Brain,C6,3T3,Hela cell lysates using PEF1 Rabbit mAb(1:1000 diluted). Predicted band size:30kDa. Observed band size:30kDa.)
Western Blot (WB)
(Western blot detection of PEF1 in K562,Rat Brain,C6,3T3,Hela cell lysates using PEF1 Rabbit mAb(1:1000 diluted). Predicted band size:30kDa. Observed band size:30kDa.)
Related Product Information for anti-PEF1 antibody
This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.[provided by RefSeq, May 2010]
Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats (PubMed:27716508). In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification (PubMed:27716508). Its role in the heterodimer formed with PDCD6 is however unclear: some evidences show that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium (PubMed:27716508). Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (PubMed:11278427). Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31.
Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats (PubMed:27716508). In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification (PubMed:27716508). Its role in the heterodimer formed with PDCD6 is however unclear: some evidences show that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium (PubMed:27716508). Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (PubMed:11278427). Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,381 Da
NCBI Official Full Name
peflin
NCBI Official Synonym Full Names
penta-EF-hand domain containing 1
NCBI Official Symbol
PEF1
NCBI Official Synonym Symbols
ABP32; PEF1A
NCBI Protein Information
peflin; PEF protein with a long N-terminal hydrophobic domain; penta-EF hand domain containing 1
UniProt Protein Name
Peflin
Protein Family
UniProt Gene Name
PEF1
UniProt Synonym Gene Names
ABP32
UniProt Entry Name
PEF1_HUMAN
NCBI Description
This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.[provided by RefSeq, May 2010]
Uniprot Description
PEF1: Heterodimer; heterodimerizes with PDCD6/ALG2. Dissociates from PDCD6/ALG2 in presence of Ca(2+).
Chromosomal Location of Human Ortholog: 1p34
Cellular Component: membrane; cytoplasm
Molecular Function: protein dimerization activity; protein binding; protein heterodimerization activity; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding
Biological Process: proteolysis; response to calcium ion
Research Articles on PEF1
Similar Products
Product Notes
The PEF1 pef1 (Catalog #AAA4752946) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PEF1 Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PEF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:1000-1:5000 FC/FACS: 1:20 IP: 1:20-1:50. Researchers should empirically determine the suitability of the PEF1 pef1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PEF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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