Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using BRSK1 antibody.)
Rabbit anti-Human, Mouse BRSK1 Polyclonal Antibody | anti-BRSK1 antibody
BRSK1 Antibody
Gene Names
BRSK1; hSAD1
Reactivity
Human, Mouse
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
BRSK1; Polyclonal Antibody; BRSK1 Antibody; EC 2.7.11.1; SAD1 kinase; Serine/threonine kinase SAD-B; anti-BRSK1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total BRSK1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
778
Applicable Applications for anti-BRSK1 antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500~1:3000
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from internal of human BRSK1.
Target Name
BRSK1
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using BRSK1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using BRSK1 antibody.)
Related Product Information for anti-BRSK1 antibody
Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.Lizcano J.M., EMBO J. 23:833-843(2004).Lu R., J. Biol. Chem. 279:31164-31170(2004).Nagase T., DNA Res. 8:85-95(2001).
Product Categories/Family for anti-BRSK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,782 Da
NCBI Official Full Name
serine/threonine-protein kinase BRSK1
NCBI Official Synonym Full Names
BR serine/threonine kinase 1
NCBI Official Symbol
BRSK1
NCBI Official Synonym Symbols
hSAD1
NCBI Protein Information
serine/threonine-protein kinase BRSK1
UniProt Protein Name
Serine/threonine-protein kinase BRSK1
Protein Family
UniProt Gene Name
BRSK1
UniProt Synonym Gene Names
KIAA1811; SAD1; SADB; BR serine/threonine-protein kinase 1; SAD1 homolog; hSAD1
UniProt Entry Name
BRSK1_HUMAN
Uniprot Description
Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.
Research Articles on BRSK1
1. BRSK1 is a novel tumor suppressor in breast cancer which inversely correlated with Jab1 expression.
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Product Notes
The BRSK1 brsk1 (Catalog #AAA9405368) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRSK1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BRSK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500~1:3000. Researchers should empirically determine the suitability of the BRSK1 brsk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRSK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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