Rabbit anti-Human BTAF1 Polyclonal Antibody | anti-BTAF1 antibody

BTAF1 Antibody

Cat. Num. AAA9404494

• Gene Names: BTAF1; MOT1; TAF172; TAFII170; TAF(II)170
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: BTAF1; Polyclonal Antibody; BTAF1 Antibody; MOT1; TAF172; TAFII170; TAF(II)170; anti-BTAF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total BTAF1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1849

• Applicable Applications for anti-BTAF1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (CHIP)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200; Immunoprecipitation: 1:20 - 1:100; Chromatin immunoprecipitation: 1:20 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human BTAF1.
• Target Name: BTAF1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using BTAF1 antibody.)

Related Product Information for anti-BTAF1 antibody

This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters.

Product Categories/Family for anti-BTAF1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 27477070
• NCBI GeneID: 9044
• NCBI Accession #: NP_003963.1
• NCBI GenBank Nucleotide #: NM_003972.2
• UniProt Accession #: O14981; O43578; B4E0W6
• Molecular Weight: 76,419 Da
• NCBI Official Full Name: TATA-binding protein-associated factor 172
• NCBI Official Synonym Full Names: BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa
• NCBI Official Symbol: BTAF1
• NCBI Official Synonym Symbols: MOT1; TAF172; TAFII170; TAF(II)170
• NCBI Protein Information: TATA-binding protein-associated factor 172
• UniProt Protein Name: TATA-binding protein-associated factor 172
• Protein Family: TATA-binding protein-associated factor
• UniProt Gene Name: BTAF1
• UniProt Synonym Gene Names: TAF172; TAF-172
• UniProt Entry Name: BTAF1_HUMAN

NCBI Description

This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters. [provided by RefSeq, Sep 2011]

Uniprot Description

TAF172: Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner. Associates with TBP to form B-TFIID. Binds DRAP1. Belongs to the SNF2/RAD54 helicase family.

Protein type: Helicase; EC 3.6.4.-; Transcription initiation complex; EC 3.6.1.-

Chromosomal Location of Human Ortholog: 10q22-q23

Cellular Component: nucleus

Molecular Function: DNA binding; helicase activity; transcription factor activity; ATP binding

Biological Process: metabolic process; negative regulation of transcription, DNA-dependent