Mouse anti-Human HSP70 Monoclonal Antibody | anti-HSP70 antibody

HSP70 Antibody

Cat. Num. AAA9383251

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity-Chromatography
• Synonyms: HSP70; Monoclonal Antibody; HSP70 Antibody; anti-HSP70 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Specificity: HSP70 anitbody detects endogenous levels of total HSP70.
• Purity/Purification: Affinity-Chromatography
• Concentration: 1.0mg/ml (varies by lot)

• Applicable Applications for anti-HSP70 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1/10000, WB: 1/500-1/2000, IHC:1/200-1/1000, ICC: 1/200-1/1000; ; Important: For Western Blots, incubate membrane with diluted antibody in 5% w/v milk, 1x TBS, 0.1% Tween (R) 20 at 4 degree C with gentle shaking, overnight.
• Subcellular Location: Cytoskeleton; Cytosol; Endoplasmic reticulum; Extracellular region or secreted; Mitochondrion; Nucleus.
• Tissue Specificity: By heat shock
• Similarity: The N-terminal nucleotide binding domain (NBD) (also known as the ATPase domain) is responsible for binding and hydrolyzing ATP. The C-terminal substrate-binding domain (SBD) (also known as peptide-binding domain) binds to the client/substrate proteins. The two domains are allosteric ally coupled so that, when ATP is bound to the NBD, the SBD binds relatively weakly to clients. When ADP is bound in the NBD, a conformational change enhances the affinity of the SBD for client proteins. Belongs to the heat shock protein 70 family.

Western Blot

(Western Blot analysis using HSP70 mouse mAb against Hela (1) cell lysate.)

Immunohistochemistry

(Immunohistochemical analysis of paraffin embedded human breast cancer using HSP70 mouse mAb with DAB staining.)

Imunofluorescence

(Imunofluorescence analysis of NIH/3T3 cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.)

Related Product Information for anti-HSP70 antibody

This intron less gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

NCBI and Uniprot Product Information

• UniProt Accession #: P0DMV8
• Molecular Weight: 70kDa
• Protein Family: HSP70 co-chaperone

NCBI Description

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]