anti-Human Hsp70 Monoclonal Antibody | anti-Hsp70 antibody

Anti-Hsp70 (N-term) Mouse mAb

Cat. Num. AAA475097

• Gene Names: HSPA1B; HSP70-2; HSP70.2; HSP70-1B
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry
• Purity: Affinity Purified
• Synonyms: Hsp70; Monoclonal Antibody; Anti-Hsp70 (N-term) Mouse mAb; DAQB 147D11.1 001; FLJ54303; FLJ54370; FLJ54392; FLJ54408; FLJ75127; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A/1B; heat shock 70kDa protein 1A; Heat shock 70kDa protein 1B; Heat shock induced protein; heat shock protein 70; HSP70 1; HSP70 2; HSP70-1/HSP70-2; HSP70-1A; HSP70.1; HSP70.1/HSP70.2; HSP70I; HSP71_HUMAN; HSP72; HSPA1; HSPA1A; HSPA1B; XXbac BCX40G17.3 001; anti-Hsp70 antibody

• Host: Mouse; Source: Human
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2D4-F7-C11
• Purity/Purification: Affinity Purified
• Form/Format: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%, glycerol
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-Hsp70 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1000; ICC: 1:300
• Immunogen: Purified recombinant human Hsp70 (N-terminus) protein fragments expressed in E Coli.
• Sensitivity: This antibody detects endogenous levels of Hsp70 (N-terminus) and does not cross-react with related proteins.
• Sequence Similarities: Belongs to the heat shock protein 70 family
• Subunit Structure: Component of a complex composed at least of CATSPER1, CATSPERB, CATSPERG and HSPA1B. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with TSC2. Interacts with IRAK1BP1. Interacts with TERT; the interaction occurs in the absence of the RNA component, TERC, and dissociates once the TERT complex has formed. Interacts with DNAJC7. Interacts with CHCHD3.
• Subcellular Location: Cytoplasm

Western Blot (WB)

(Western blot detection of Hsp70 (N-terminus) in Heat shcok Hela, Hela and HepG2 lysates using Hsp70 (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 70KDa.Observed band size: 70KDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry stain of Hela using Hsp70 (N-terminus) mouse mAb (1:300).)

Related Product Information for anti-Hsp70 antibody

Entrez Summary: This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 147744565
• NCBI GeneID: 3304
• NCBI Accession #: P08107.5
• NCBI GenBank Nucleotide #: NM_005345
• UniProt Accession #: P08107; P0DMV9
• Molecular Weight: 70
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 1B
• NCBI Official Symbol: HSPA1B
• NCBI Official Synonym Symbols: HSP70-2; HSP70.2; HSP70-1B
• NCBI Protein Information: heat shock 70 kDa protein 1B
• Protein Family: HSP70 co-chaperone

NCBI Description

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]